DNA replication timing of the human β-globin domain is controlled by histone modification at the origin

Alon Goren, Amalia Tabib, Merav Hecht, Howard Cedar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

The human β-globin genes constitute a large chromosomal domain that is developmentally regulated. In non-erythroid cells, these genes replicate late in S phase, while in erythroid cells, replication is early. The replication origin is packaged with acetylated histones in erythroid cells, yet is associated with deacetylated histones in nonerythroid cells. Recruitment of histone acetylases to this origin brings about a transcription-independent shift to early replication in lymphocytes. In contrast, tethering of a histone deacetylase in erythroblasts causes a shift to late replication. These results suggest that histone modification at the origin serves as a binary switch for controlling replication timing.

Original languageAmerican English
Pages (from-to)1319-1324
Number of pages6
JournalGenes and Development
Volume22
Issue number10
DOIs
StatePublished - 15 May 2008

Keywords

  • Chromatin structure
  • Development
  • Epigenetics
  • Gene expression
  • Replication timing

Fingerprint

Dive into the research topics of 'DNA replication timing of the human β-globin domain is controlled by histone modification at the origin'. Together they form a unique fingerprint.

Cite this