DNA sequence of SSCP haplotypes at the bovine growth hormone (bGH) gene

A. Lagziel, M. Soller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Previous studies using SSCP and PCR-RFLP methodologies uncovered nine polymorphic sites within the bGH gene, defining eight intragenic haplotypes falling into two main groups. In the present study we report the DNA sequence of these eight haplotypes. A total of 1494 bp were sequenced uncovering a total of 12 sequence variants. Hap lotypes within groups differed among themselves at one or two sites; compared across groups, haplotypes of the two groups differed consistently at six sites, each of which was monomorphic within the respective groups. This comes to 4 differentiating sites per kb, suggesting that the two haplotype groups began to diverge about 400 000 years ago. This corresponds approximately to the estimated time of divergence of the Bos taurus and Bos indicus lineages, raising the possibility, supported by other evidence, that the two haplotype classes represent taurine and indicine haplotypes, respectively. Nucleotide sequence divergence of taurine and indicine genomes of this magnitude has far reaching implications with respect to QTL mapping and marker assisted selection in breeds derived from taurine x indicine crosses.

Original languageEnglish
Pages (from-to)362-365
Number of pages4
JournalAnimal Genetics
Volume30
Issue number5
DOIs
StatePublished - Oct 1999

Keywords

  • Bovine
  • DNA sequence
  • Growth hormone
  • Haplotypes
  • Molecular clock

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