DNase I sensitivity in facultative and constitutive heterochromatin

Karl Sperling*, Bat Sheva Kerem, Ruth Goitein, Veronika Kottusch, Howard Cedar, Menashe Marcus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


In situ nick translation allows the detection of DNase I sensitive and insensitive regions in fixed mammalian mitotic chromosomes. We have determined the difference in DNase I sensitivity between the active and inactive X chromosomes in Microtus agrestis (rodent) cells, along both their euchromatic and constitutive heterochromatic regions. In addition, we analysed the DNase I sensitivity of the constitutive heterochromatic regions in mouse chromosomes. In Microtus agrestis female cells the active X chromosome is sensitive to DNase I along its euchromatic region while the inactive X chromosome is insensitive except for an early replicating region at its distal end. The late replicating constitutive heterochromatic regions, however, in both the active and inactive X chromosome are sensitive to DNase I. In mouse cells on the other hand, the constitutive heterochromatin is insensitive to DNase I both in mitotic chromosomes and interphase nuclei.

Original languageAmerican English
Pages (from-to)38-42
Number of pages5
Issue number1
StatePublished - Jan 1985


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