DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

Tamara Niazov; Valeri Pavlov; Yi Xiao; Ron Gill; Itamar Willner

doi:10.1021/nl0491428

DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

Tamara Niazov, Valeri Pavlov, Yi Xiao, Ron Gill, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

276 Scopus citations

Abstract

DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H₂O ₂, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10^-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.

Original language	English
Pages (from-to)	1683-1687
Number of pages	5
Journal	Nano Letters
Volume	4
Issue number	9
DOIs	https://doi.org/10.1021/nl0491428
State	Published - Sep 2004

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title = "DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity",

abstract = "DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H2O 2, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.",

author = "Tamara Niazov and Valeri Pavlov and Yi Xiao and Ron Gill and Itamar Willner",

year = "2004",

month = sep,

doi = "10.1021/nl0491428",

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AU - Gill, Ron

AU - Willner, Itamar

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N2 - DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H2O 2, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.

AB - DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H2O 2, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.

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DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

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