DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

Tamara Niazov; Valeri Pavlov; Yi Xiao; Ron Gill; Itamar Willner

doi:10.1021/nl0491428

DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

Tamara Niazov
, Valeri Pavlov
, Yi Xiao
, Ron Gill
, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

281 Scopus citations

Abstract

DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H₂O ₂, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10^-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.

Original language	English
Pages (from-to)	1683-1687
Number of pages	5
Journal	Nano Letters
Volume	4
Issue number	9
DOIs	https://doi.org/10.1021/nl0491428
State	Published - Sep 2004

Access to Document

10.1021/nl0491428

Cite this

@article{b5121d1852da4966ad386b81b6c0c46b,

title = "DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity",

abstract = "DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H2O 2, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.",

author = "Tamara Niazov and Valeri Pavlov and Yi Xiao and Ron Gill and Itamar Willner",

year = "2004",

month = sep,

doi = "10.1021/nl0491428",

language = "אנגלית",

volume = "4",

pages = "1683--1687",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "9",

}

TY - JOUR

T1 - DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

AU - Niazov, Tamara

AU - Pavlov, Valeri

AU - Xiao, Yi

AU - Gill, Ron

AU - Willner, Itamar

PY - 2004/9

Y1 - 2004/9

N2 - DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H2O 2, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.

AB - DNAzyme-functionalized Au-NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H2O 2, and luminol, the generation of chemiluminescence. For DNA analysis, a nucleic acid unit complementary to the analyzed DNA is tethered to the DNAzyme structure associated with the Au-NPs. For telomerase activity, a nucleic acid complementary to the telomer repeat units generated on the surface is tethered to the DNAzyme structure associated with the Au-NPs. The detection limit for the detection of DNA is 1 × 10-10 M. The method enables the detection of telomerase activity originating from 1000 HeLa cells.

UR - https://www.scopus.com/pages/publications/4644288203

U2 - 10.1021/nl0491428

DO - 10.1021/nl0491428

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:4644288203

SN - 1530-6984

VL - 4

SP - 1683

EP - 1687

JO - Nano Letters

JF - Nano Letters

IS - 9

ER -

DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity

Abstract

Access to Document

Other files and links

Fingerprint

Cite this