Does metabolic water control the phosphate oxygen isotopes of microbial cells?

Tal Weiner, Federica Tamburini, Nir Keren, Jonathan Keinan, Alon Angert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The oxygen isotopes ratio (δ18O) of microbial cell water strongly controls the δ18O of cell phosphate and of other oxygen-carrying moieties. Recently it was suggested that the isotopic ratio in cell water is controlled by metabolic water, which is the water produced by cellular respiration. This potentially has important implications for paleoclimate reconstruction, and for measuring microbial carbon use efficiency with the 18O-water method. Carbon use efficiency strongly controls soil organic matter preservation. Here, we directly tested the effect of metabolic water on microbial cells, by conducting experiments with varying the δ18O of headspace O2 and the medium water, and by measuring the δ18O of cell phosphate. The latter is usually assumed to be in isotopic equilibrium with the cell’s water. Our results showed no correlation between the δ18O of O2 and that of the cell phosphate, contradicting the hypothesis that metabolic water is an important driver of δ18O of microbial cell water. However, our labeled 18O water experiments indicated that only 43% of the oxygen in the cell’s phosphate is derived from equilibration with the medium water, during late-log to early-stationary growing phase. This could be explained by the isotopic effects of intra-and extra-cellular hydrolysis of organic compounds containing phosphate.

Original languageAmerican English
Article number1277349
JournalFrontiers in Microbiology
Volume14
DOIs
StatePublished - 2023

Bibliographical note

Publisher Copyright:
Copyright © 2023 Weiner, Tamburini, Keren, Keinan and Angert.

Keywords

  • ambient water
  • carbon use efficiency
  • metabolic water
  • microbial cells
  • oxygen stable isotopes

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