TY - JOUR
T1 - Duration of antigen expression in vivo following DNA immunization modifies the magnitude, contraction, and secondary responses of CD8+ T lymphocytes
AU - Hovav, Avi Hai
AU - Panas, Michael W.
AU - Rahman, Shaila
AU - Sircar, Piya
AU - Gillard, Geoffrey
AU - Cayabyab, Mark J.
AU - Letvin, Norman L.
PY - 2007/11/15
Y1 - 2007/11/15
N2 - The duration of Ag expression in vivo has been reported to have a minimal impact on both the magnitude and kinetics of contraction of a pathogen-induced CD8+ T cell response. In this study, we controlled the duration of Ag expression by excising the ear pinnae following intradermal ear pinnae DNA immunization. This resulted in decreased magnitude, accelerated contraction and differentiation, and surprisingly greater secondary CD8+ T cell responses. Furthermore, we found delayed and prolonged Ag presentation in the immunized mice; however, this presentation was considerably decreased when the depot Ag was eliminated. These findings suggest that the magnitude and the contraction phase of the CD8+ T cell response following intradermal DNA immunization is regulated by the duration rather than the initial exposure to Ag.
AB - The duration of Ag expression in vivo has been reported to have a minimal impact on both the magnitude and kinetics of contraction of a pathogen-induced CD8+ T cell response. In this study, we controlled the duration of Ag expression by excising the ear pinnae following intradermal ear pinnae DNA immunization. This resulted in decreased magnitude, accelerated contraction and differentiation, and surprisingly greater secondary CD8+ T cell responses. Furthermore, we found delayed and prolonged Ag presentation in the immunized mice; however, this presentation was considerably decreased when the depot Ag was eliminated. These findings suggest that the magnitude and the contraction phase of the CD8+ T cell response following intradermal DNA immunization is regulated by the duration rather than the initial exposure to Ag.
UR - http://www.scopus.com/inward/record.url?scp=38449105825&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.179.10.6725
DO - 10.4049/jimmunol.179.10.6725
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C2 - 17982062
AN - SCOPUS:38449105825
SN - 0022-1767
VL - 179
SP - 6725
EP - 6733
JO - Journal of Immunology
JF - Journal of Immunology
IS - 10
ER -