TY - JOUR
T1 - Dynamics of cell area and force during spreading
AU - Brill-Karniely, Yifat
AU - Nisenholz, Noam
AU - Rajendran, Kavitha
AU - Dang, Quynh
AU - Krishnan, Ramaswamy
AU - Zemel, Assaf
N1 - Publisher Copyright:
© 2014 Biophysical Society.
PY - 2014/12/16
Y1 - 2014/12/16
N2 - Experiments on human pulmonary artery endothelial cells are presented to show that cell area and the force exerted on a substrate increase simultaneously, but with different rates during spreading; rapid-force increase systematically occurred several minutes past initial spreading. We examine this theoretically and present three complementary mechanisms that may accompany the development of lamellar stress during spreading and underlie the observed behavior. These include: 1), the dynamics of cytoskeleton assembly at the cell basis; 2), the strengthening of acto-myosin forces in response to the generated lamellar stresses; and 3), the passive strain-stiffening of the cytoskeleton.
AB - Experiments on human pulmonary artery endothelial cells are presented to show that cell area and the force exerted on a substrate increase simultaneously, but with different rates during spreading; rapid-force increase systematically occurred several minutes past initial spreading. We examine this theoretically and present three complementary mechanisms that may accompany the development of lamellar stress during spreading and underlie the observed behavior. These include: 1), the dynamics of cytoskeleton assembly at the cell basis; 2), the strengthening of acto-myosin forces in response to the generated lamellar stresses; and 3), the passive strain-stiffening of the cytoskeleton.
UR - http://www.scopus.com/inward/record.url?scp=84920122092&partnerID=8YFLogxK
U2 - 10.1016/j.bpj.2014.10.049
DO - 10.1016/j.bpj.2014.10.049
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 25517168
AN - SCOPUS:84920122092
SN - 0006-3495
VL - 107
SP - L37-L40
JO - Biophysical Journal
JF - Biophysical Journal
IS - 12
ER -