TY - JOUR
T1 - Dynamics of Chromatin and Transcription during Transient Depletion of the RSC Chromatin Remodeling Complex
AU - Klein-Brill, Avital
AU - Joseph-Strauss, Daphna
AU - Appleboim, Alon
AU - Friedman, Nir
N1 - Publisher Copyright:
© 2018 The Author(s)
PY - 2019/1/2
Y1 - 2019/1/2
N2 - Nucleosome organization has a key role in transcriptional regulation, yet the precise mechanisms establishing nucleosome locations and their effect on transcription are unclear. Here, we use an induced degradation system to screen all yeast ATP-dependent chromatin remodelers. We characterize how rapid clearance of the remodeler affects nucleosome locations. Specifically, depletion of Sth1, the catalytic subunit of the RSC (remodel the structure of chromatin) complex, leads to rapid fill-in of nucleosome-free regions at gene promoters. These changes are reversible upon reintroduction of Sth1 and do not depend on DNA replication. RSC-dependent nucleosome positioning is pivotal in maintaining promoters of lowly expressed genes free from nucleosomes. In contrast, we observe that upon acute stress, the RSC is not necessary for the transcriptional response. Moreover, RSC-dependent nucleosome positions are tightly related to usage of specific transcription start sites. Our results suggest organizational principles that determine nucleosome positions with and without RSC and how these interact with the transcriptional process.
AB - Nucleosome organization has a key role in transcriptional regulation, yet the precise mechanisms establishing nucleosome locations and their effect on transcription are unclear. Here, we use an induced degradation system to screen all yeast ATP-dependent chromatin remodelers. We characterize how rapid clearance of the remodeler affects nucleosome locations. Specifically, depletion of Sth1, the catalytic subunit of the RSC (remodel the structure of chromatin) complex, leads to rapid fill-in of nucleosome-free regions at gene promoters. These changes are reversible upon reintroduction of Sth1 and do not depend on DNA replication. RSC-dependent nucleosome positioning is pivotal in maintaining promoters of lowly expressed genes free from nucleosomes. In contrast, we observe that upon acute stress, the RSC is not necessary for the transcriptional response. Moreover, RSC-dependent nucleosome positions are tightly related to usage of specific transcription start sites. Our results suggest organizational principles that determine nucleosome positions with and without RSC and how these interact with the transcriptional process.
KW - RISC
KW - chromatin
KW - chromatin remodeling
KW - transcription
KW - transcription initiation
UR - http://www.scopus.com/inward/record.url?scp=85059094454&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2018.12.020
DO - 10.1016/j.celrep.2018.12.020
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C2 - 30605682
AN - SCOPUS:85059094454
SN - 2211-1247
VL - 26
SP - 279-292.e5
JO - Cell Reports
JF - Cell Reports
IS - 1
ER -