Dynamics of replication-independent histone turnover in budding yeast

Michael F. Dion; Tommy Kaplan; Minkyu Kim; Stephen Buratowski; Nir Friedman; Oliver J. Rando

doi:10.1126/science.1134053

Dynamics of replication-independent histone turnover in budding yeast

Michael F. Dion, Tommy Kaplan, Minkyu Kim, Stephen Buratowski, Nir Friedman, Oliver J. Rando^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

451 Scopus citations

Abstract

Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (∼265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.

Original language	English
Pages (from-to)	1405-1408
Number of pages	4
Journal	Science
Volume	315
Issue number	5817
DOIs	https://doi.org/10.1126/science.1134053
State	Published - 9 Mar 2007

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health grants GM 16767, AM 17884, and 2S07RR0712213. C. P. Scholes was the recipient of the National Institutes of Health Research Career Development Award No. AM00274. Receivedfor publication 14 December 1982 and infinalform 9 August 1983.

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abstract = "Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (∼265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.",

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AU - Kim, Minkyu

AU - Buratowski, Stephen

AU - Friedman, Nir

AU - Rando, Oliver J.

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Dynamics of replication-independent histone turnover in budding yeast

Abstract

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