Dynamics of the cytosolic chelatable iron pool of K562 cells

William Breuer; Silvina Epsztejn; Z. Ioav Cabantchik

doi:10.1016/0014-5793(96)00190-1

Dynamics of the cytosolic chelatable iron pool of K562 cells

William Breuer^*, Silvina Epsztejn, Z. Ioav Cabantchik

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol, We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350 nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t( 1/2 )s Of 27, 65-88 and ≤17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.

Original language	English
Pages (from-to)	304-308
Number of pages	5
Journal	FEBS Letters
Volume	382
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(96)00190-1
State	Published - 18 Mar 1996

Keywords

Calcein
Chelators
Fluorescence
Iron
Oxidative stress

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10.1016/0014-5793(96)00190-1

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N2 - The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol, We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350 nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t( 1/2 )s Of 27, 65-88 and ≤17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.

AB - The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol, We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350 nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t( 1/2 )s Of 27, 65-88 and ≤17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.

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KW - Oxidative stress

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Dynamics of the cytosolic chelatable iron pool of K562 cells

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Keywords

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