TY - JOUR
T1 - Dynein light chain binding to a 3'-untranslated sequence mediates parathyroid hormone mRNA association with microtubules
AU - Epstein, Eyal
AU - Sela-Brown, Alin
AU - Ringel, Israel
AU - Kilav, Rachel
AU - King, Stephen M.
AU - Benashski, Sharon E.
AU - Yisraeli, Joel K.
AU - Silver, Justin
AU - Naveh-Many, Tally
PY - 2000/2
Y1 - 2000/2
N2 - The 3'-untranslated region (UTR) of mRNAs binds proteins that determine mRNA stability and localization. The 3'-UTR of parathyroid hormone (PTH) mRNA specifically binds cytoplasmic proteins. We screened an expression library for proteins that bind the PTH mRNA 3'-UTR, and the sequence of 1 clone was identical to that of the dynein light chain LC8, a component of the dynein complexes that translocate cytoplasmic components along microtubules. Recombinant LC8 binds PTH mRNA 3'-UTR, as shown by RNA electrophoretic mobility shift assay. We showed that PTH mRNA colocalizes with microtubules in the parathyroid gland, as well as with a purified microtubule preparation from calf brain, and that this association was mediated by LC8. To our knowledge, this is the first report of a dynein complex protein binding an mRNA. The dynein complex may be the motor that is responsible for transporting mRNAs to specific locations in the cytoplasm and for the consequent is asymmetric distribution of translated proteins in the cell.
AB - The 3'-untranslated region (UTR) of mRNAs binds proteins that determine mRNA stability and localization. The 3'-UTR of parathyroid hormone (PTH) mRNA specifically binds cytoplasmic proteins. We screened an expression library for proteins that bind the PTH mRNA 3'-UTR, and the sequence of 1 clone was identical to that of the dynein light chain LC8, a component of the dynein complexes that translocate cytoplasmic components along microtubules. Recombinant LC8 binds PTH mRNA 3'-UTR, as shown by RNA electrophoretic mobility shift assay. We showed that PTH mRNA colocalizes with microtubules in the parathyroid gland, as well as with a purified microtubule preparation from calf brain, and that this association was mediated by LC8. To our knowledge, this is the first report of a dynein complex protein binding an mRNA. The dynein complex may be the motor that is responsible for transporting mRNAs to specific locations in the cytoplasm and for the consequent is asymmetric distribution of translated proteins in the cell.
UR - http://www.scopus.com/inward/record.url?scp=0034000792&partnerID=8YFLogxK
U2 - 10.1172/JCI8557
DO - 10.1172/JCI8557
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C2 - 10683380
AN - SCOPUS:0034000792
SN - 0021-9738
VL - 105
SP - 505
EP - 512
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 4
ER -