TY - JOUR
T1 - Early chick embryonic cells can form clones in agarose cultures
AU - Mitrani, Eduardo
PY - 1984/5
Y1 - 1984/5
N2 - Early chick embryonic cells prior to the formation of the primitive streak, have been cultured in a two-layer soft-agarose system. Single, primary cells when grown in this system were capable of producing colonies ranging in size from 30 to 100 cells. The plating efficiency varied between 1 and 5% and the colonies remained viable for about 2 weeks. We believe this is the first report of normal, non-passaged cells which show anchorage-independent growth properties by forming colonies in a standard agarose culture in the absence of additional factors. The importance of being able to use normal monoclonal embryonic cell populations in studying early developmental processes is also discussed.
AB - Early chick embryonic cells prior to the formation of the primitive streak, have been cultured in a two-layer soft-agarose system. Single, primary cells when grown in this system were capable of producing colonies ranging in size from 30 to 100 cells. The plating efficiency varied between 1 and 5% and the colonies remained viable for about 2 weeks. We believe this is the first report of normal, non-passaged cells which show anchorage-independent growth properties by forming colonies in a standard agarose culture in the absence of additional factors. The importance of being able to use normal monoclonal embryonic cell populations in studying early developmental processes is also discussed.
UR - http://www.scopus.com/inward/record.url?scp=0021345956&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(84)90238-6
DO - 10.1016/0014-4827(84)90238-6
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C2 - 6714316
AN - SCOPUS:0021345956
SN - 0014-4827
VL - 152
SP - 148
EP - 153
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -