Ectopic induction of cartilage and bone by water‐soluble proteins from bovine bone using a polyanhydride delivery vehicle

Paul A. Lucas*, Cato Laurencin, Glenn T. Syftestad, Abraham Domb, Victor M. Goldberg, Arnold I. Caplan, Robert Langer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Controlled release delivery vehicles for water‐soluble osteogenic proteins from demineralized bovine bone matrix were constructed using polyanhydride polymers. The water‐soluble proteins were isolated from a 4 M guanidine hydrochloride extract of bone matrix. The water‐soluble proteins possessed Chondrogenic Stimulating Activity (CSA) when tested in stage 24 chick limb bud cell cultures, but were incapable of inducing cartilage or bone in vivo when implanted intramuscularly into mice by themselves. The polyanhydride polymers alone were also incapable of inducting ectopic cartilage or bone. However, when the water‐soluble proteins were incorporated into the polymeric delivery vehicle, the combination was capable of inducing cartilage and bone up to 50% of the time. These results demonstrate that it is possible to use polyanhydride polymers as controlled‐release delivery vehicles for soluble bioactive factors that interact with a local cell population.

Original languageEnglish
Pages (from-to)901-911
Number of pages11
JournalJournal of Biomedical Materials Research
Volume24
Issue number7
DOIs
StatePublished - Jul 1990
Externally publishedYes

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