Effect of Azospirillum brasilense coinoculated with Rhizobium on Phaseolus vulgaris flavonoids and Nod factor production under salt stress

Marta S. Dardanelli*, Francisco J. Fernández de Córdoba, M. Rosario Espuny, Miguel A. Rodríguez Carvajal, María E. Soria Díaz, Antonio M. Gil Serrano, Yaacov Okon, Manuel Megías

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

205 Scopus citations

Abstract

The effects of salt upon Azospirillum brasilense strain Cd on plant growth, nodulation, flavonoid and lipochitooligosaccharide (LCOs-Nod factor) production, were sequentially followed after 4, 7 and 14 days during a Rhizobium-Phaseolus vulgaris cv. Negro Jamapa interaction, in a hydroponics growth system. Azospirillum brasilense promoted root branching in bean seedling roots and increased secretion of nod-gene-inducing flavonoid species, as detected by high-performance liquid chromatography (HPLC). The results also support that A. brasilense allows a longer, more persistent exudation of flavonoids by bean roots. A general positive effect of Azospirillum-Rhizobium coinoculation on the expression of nod-genes by Rhizobium tropici CIAT899 and Rhizobium etli ISP42, and on nodulation factor patterns, was observed in the presence of root exudates. The negative effects obtained under salt stress on nod-gene expression and on Nod factors' appearance were relieved in coinoculated plants.

Original languageEnglish
Pages (from-to)2713-2721
Number of pages9
JournalSoil Biology and Biochemistry
Volume40
Issue number11
DOIs
StatePublished - Nov 2008

Keywords

  • Abiotic stress
  • Azospirillum brasilense
  • Coinoculation
  • Flavonoids
  • Nod factors
  • nod-gene expression
  • Rhizobium etli
  • Rhizobium tropici

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