Effect of Azospirillum brasilense inoculation on rhizobacterial communities analyzed by denaturing gradient gel electrophoresis and automated ribosomal intergenic spacer analysis

Anat Lerner, Yoav Herschkovitz, Ezekiel Baudoin, Sylvie Nazaret, Yvan Moenne-Loccoz, Yaacov Okon, Edouard Jurkevitch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Nucleic acid-based techniques allow the exploration of microbial communities in the environments such as the rhizosphere. Azospirillum brasilense, a plant growth promoting rhizobacterium (PGPR), causes morphological changes in the plant root system. These changes in root physiology may indirectly affect the microbial diversity of the rhizosphere. In this study, the changes in the rhizobacterial structure following A. brasilense inoculation of maize (Zea mays) plants was examined by PCR-denaturating gradient gel electrophoresis (DGGE) and automated ribosomal intergenic spacer analysis (ARISA), using two universal primers sets for the 16S rRNA gene, and an intergenic 16S-23S rDNA primer set, respectively. Similar results were obtained when using either ARISA or DGGE performed with these different primer sets, and analyzed by different statistical methods: no prominent effect of A. brasilense inoculation was observed on the bacterial communities of plant roots grown in two different soils and in different growth systems. In contrast, plant age caused significant shifts in the bacterial populations.

Original languageAmerican English
Pages (from-to)1212-1218
Number of pages7
JournalSoil Biology and Biochemistry
Volume38
Issue number6
DOIs
StatePublished - Jun 2006
Externally publishedYes

Keywords

  • A. brasilense
  • ARISA
  • Eubacterial primer sets
  • Inoculation
  • PCR-DGGE
  • PGPR

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