Effect of chlorhexidine on molecular weight distribution of fructans produced by fructosyltransferase in solution and immobilized on surface

Ramona Rozen; Gilad Bachrach; Batia Zaks; Moshe Bronshteyn; Itzhak Gedalia; Doron Steinberg

doi:10.1016/S0008-6215(02)00468-8

Effect of chlorhexidine on molecular weight distribution of fructans produced by fructosyltransferase in solution and immobilized on surface

Ramona Rozen, Gilad Bachrach, Batia Zaks, Moshe Bronshteyn, Itzhak Gedalia, Doron Steinberg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The effect of chlorhexidine (CHX), a potent antibacterial agent, was tested on the molecular weight distribution (MWD) of fructans synthesized by cell-free fructosyltransferase (FTF) in solution in comparison to FTF immobilized onto hydroxyapatite (HA). Size-exclusion chromatography (SEC) analysis has shown that cell-free FTF, both in solution and immobilized on HA, produces both low MW (1.9-2.2 kDa) and high MW (913-1047 kDa) fructans. CHX at a concentration of 0.02% altered the MWD of the fructans by reducing the polydispersity ratio and changing the MWD of the fructans synthesized both by immobilized FTF and by FTF in solution. These changes of the fructans in the presence of CHX adds a new prospective to the anticaries effect of CHX in addition to its antibacterial properties.

Original language	English
Pages (from-to)	571-575
Number of pages	5
Journal	Carbohydrate Research
Volume	338
Issue number	6
DOIs	https://doi.org/10.1016/S0008-6215(02)00468-8
State	Published - 14 Mar 2003
Externally published	Yes

Keywords

Biofilm
Chlorhexidine
Fructans
Fructosyltransferase
Hydroxyapatite
I mmobilization

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10.1016/S0008-6215(02)00468-8

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title = "Effect of chlorhexidine on molecular weight distribution of fructans produced by fructosyltransferase in solution and immobilized on surface",

abstract = "The effect of chlorhexidine (CHX), a potent antibacterial agent, was tested on the molecular weight distribution (MWD) of fructans synthesized by cell-free fructosyltransferase (FTF) in solution in comparison to FTF immobilized onto hydroxyapatite (HA). Size-exclusion chromatography (SEC) analysis has shown that cell-free FTF, both in solution and immobilized on HA, produces both low MW (1.9-2.2 kDa) and high MW (913-1047 kDa) fructans. CHX at a concentration of 0.02% altered the MWD of the fructans by reducing the polydispersity ratio and changing the MWD of the fructans synthesized both by immobilized FTF and by FTF in solution. These changes of the fructans in the presence of CHX adds a new prospective to the anticaries effect of CHX in addition to its antibacterial properties.",

keywords = "Biofilm, Chlorhexidine, Fructans, Fructosyltransferase, Hydroxyapatite, I mmobilization",

author = "Ramona Rozen and Gilad Bachrach and Batia Zaks and Moshe Bronshteyn and Itzhak Gedalia and Doron Steinberg",

year = "2003",

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doi = "10.1016/S0008-6215(02)00468-8",

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AU - Rozen, Ramona

AU - Bachrach, Gilad

AU - Zaks, Batia

AU - Bronshteyn, Moshe

AU - Gedalia, Itzhak

AU - Steinberg, Doron

PY - 2003/3/14

Y1 - 2003/3/14

N2 - The effect of chlorhexidine (CHX), a potent antibacterial agent, was tested on the molecular weight distribution (MWD) of fructans synthesized by cell-free fructosyltransferase (FTF) in solution in comparison to FTF immobilized onto hydroxyapatite (HA). Size-exclusion chromatography (SEC) analysis has shown that cell-free FTF, both in solution and immobilized on HA, produces both low MW (1.9-2.2 kDa) and high MW (913-1047 kDa) fructans. CHX at a concentration of 0.02% altered the MWD of the fructans by reducing the polydispersity ratio and changing the MWD of the fructans synthesized both by immobilized FTF and by FTF in solution. These changes of the fructans in the presence of CHX adds a new prospective to the anticaries effect of CHX in addition to its antibacterial properties.

AB - The effect of chlorhexidine (CHX), a potent antibacterial agent, was tested on the molecular weight distribution (MWD) of fructans synthesized by cell-free fructosyltransferase (FTF) in solution in comparison to FTF immobilized onto hydroxyapatite (HA). Size-exclusion chromatography (SEC) analysis has shown that cell-free FTF, both in solution and immobilized on HA, produces both low MW (1.9-2.2 kDa) and high MW (913-1047 kDa) fructans. CHX at a concentration of 0.02% altered the MWD of the fructans by reducing the polydispersity ratio and changing the MWD of the fructans synthesized both by immobilized FTF and by FTF in solution. These changes of the fructans in the presence of CHX adds a new prospective to the anticaries effect of CHX in addition to its antibacterial properties.

KW - Biofilm

KW - Chlorhexidine

KW - Fructans

KW - Fructosyltransferase

KW - Hydroxyapatite

KW - I mmobilization

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SN - 0008-6215

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JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 6

ER -

Effect of chlorhexidine on molecular weight distribution of fructans produced by fructosyltransferase in solution and immobilized on surface

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Keywords

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