Abstract
In the last decade, potential exposure of humans and animals to industrial chemicals and pesticides has been a growing concern. In the present study, di-(2-ethylhexyl) phthalate (DEHP) and mono-(2-ethylhexyl) phthalate (MEHP) were used to model the effects of endocrine-disrupting compounds and their risk in relation to early embryonic losses. Exposure of cumulus oocyte complexes during maturation to 50 μM MEHP reduced the proportion of oocytes that underwent nuclear maturation (p<0.05) and increased the proportion of apoptotic oocytes (p<0.05). Furthermore, phthalates reduced cleavage rate in the MEHP-treated group (p<0.05) and the proportion of embryos developing to the blastocyst stage in both DEHP- and MEHP-treated groups (p<0.05). The total cell count for blastocysts developing from MEHP-treated oocytes was lower than in controls (p<0.05). Exposure of oocytes to MEHP during maturation reduced (p<0.05) the expression of ASAH1 (an anti-apoptotic factor), CCNA2 (involved in cell cycle control), and POU5F1 (responsible for pluripotency) in matured oocytes. Furthermore, the reduced mRNA expression of POU5F1 and ASAH1 lasted into two-cell stage embryos (p<0.05). Phthalate-induced alterations in POU5F1, ASAH1, and CCNA2 expression might explain in part the reduced developmental competence of MEHP-treated oocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 383-396 |
| Number of pages | 14 |
| Journal | Cell Biology and Toxicology |
| Volume | 28 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2012 |
Bibliographical note
Funding Information:Acknowledgments The study has conducted in the frame-work of The Hebrew University Center of Excellence in Agriculture and Environmental Health funded by the Environment and Health Fund, Jerusalem, Israel and by the Israel Ministry of Agriculture (project #821-130-10).
Keywords
- Endocrine-disrupting compound
- Oocyte competence
- Phthalate
- Pre-implantation embryo