Effect of epigallocatechin gallate on dental biofilm of Streptococcus mutans: An in vitro study

Mor Schneider-Rayman; Doron Steinberg; Ronit Vogt Sionov; Michael Friedman; Miriam Shalish

doi:10.1186/s12903-021-01798-4

Effect of epigallocatechin gallate on dental biofilm of Streptococcus mutans: An in vitro study

Mor Schneider-Rayman^*, Doron Steinberg, Ronit Vogt Sionov, Michael Friedman, Miriam Shalish

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background: Streptococcus mutans (S. mutans) plays a major role in the formation of dental caries. The aim of this study was to examine the effect of the green tea polyphenol, epigallocatechin gallate (EGCG), on biofilm formation of S. mutans. Methods: Following exposure to increasing concentrations of EGCG, the planktonic growth was measured by optical density and the biofilm biomass was quantified by crystal violet staining. Exopolysaccharides (EPS) production was visualized by confocal scanning laser microscopy, and the bacterial DNA content was determined by quantitative polymerase chain reaction (qPCR). Gene expression of selected genes was analyzed by real time (RT)-qPCR and membrane potential was examined by flow cytometry. Results: We observed that EGCG inhibited in a dose-dependent manner both the planktonic growth and the biofilm formation of S. mutans. Significant reduction of S. mutans biofilm formation, DNA content, and EPS production was observed at 2.2–4.4 mg/ml EGCG. EGCG reduced the expression of gtfB, gtfC and ftf genes involved in EPS production, and the nox and sodA genes involved in the protection against oxidative stress. Moreover, EGCG caused an immediate change in membrane potential. Conclusions: EGCG, a natural polyphenol, has a significant inhibitory effect on S. mutans dental biofilm formation and EPS production, and thus might be a potential drug in preventing dental caries.

Original language	American English
Article number	447
Journal	BMC Oral Health
Volume	21
Issue number	1
DOIs	https://doi.org/10.1186/s12903-021-01798-4
State	Published - Dec 2021

Bibliographical note

Funding Information:
The authors would like to thank Dr. Vitaky Gutkin at The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology at the Edmond J. Safra Campus of The Hebrew University of Jerusalem for his valuable assistance with the HR-SEM analysis, and Dr. Yael Feinstein-Rotkopf at the Core Research Facility of The Hadassah Medical School for managing the Nikon Yokogawa W1 Spinning Disk microscope.

Publisher Copyright:
© 2021, The Author(s).

Keywords

Caries
Green-tea
Oral bacteria
Polyphenols

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10.1186/s12903-021-01798-4

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title = "Effect of epigallocatechin gallate on dental biofilm of Streptococcus mutans: An in vitro study",

abstract = "Background: Streptococcus mutans (S. mutans) plays a major role in the formation of dental caries. The aim of this study was to examine the effect of the green tea polyphenol, epigallocatechin gallate (EGCG), on biofilm formation of S. mutans. Methods: Following exposure to increasing concentrations of EGCG, the planktonic growth was measured by optical density and the biofilm biomass was quantified by crystal violet staining. Exopolysaccharides (EPS) production was visualized by confocal scanning laser microscopy, and the bacterial DNA content was determined by quantitative polymerase chain reaction (qPCR). Gene expression of selected genes was analyzed by real time (RT)-qPCR and membrane potential was examined by flow cytometry. Results: We observed that EGCG inhibited in a dose-dependent manner both the planktonic growth and the biofilm formation of S. mutans. Significant reduction of S. mutans biofilm formation, DNA content, and EPS production was observed at 2.2–4.4 mg/ml EGCG. EGCG reduced the expression of gtfB, gtfC and ftf genes involved in EPS production, and the nox and sodA genes involved in the protection against oxidative stress. Moreover, EGCG caused an immediate change in membrane potential. Conclusions: EGCG, a natural polyphenol, has a significant inhibitory effect on S. mutans dental biofilm formation and EPS production, and thus might be a potential drug in preventing dental caries.",

keywords = "Caries, Green-tea, Oral bacteria, Polyphenols",

author = "Mor Schneider-Rayman and Doron Steinberg and Sionov, {Ronit Vogt} and Michael Friedman and Miriam Shalish",

note = "Funding Information: The authors would like to thank Dr. Vitaky Gutkin at The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology at the Edmond J. Safra Campus of The Hebrew University of Jerusalem for his valuable assistance with the HR-SEM analysis, and Dr. Yael Feinstein-Rotkopf at the Core Research Facility of The Hadassah Medical School for managing the Nikon Yokogawa W1 Spinning Disk microscope. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1186/s12903-021-01798-4",

language = "American English",

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T2 - An in vitro study

AU - Schneider-Rayman, Mor

AU - Steinberg, Doron

AU - Sionov, Ronit Vogt

AU - Friedman, Michael

AU - Shalish, Miriam

N1 - Funding Information: The authors would like to thank Dr. Vitaky Gutkin at The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology at the Edmond J. Safra Campus of The Hebrew University of Jerusalem for his valuable assistance with the HR-SEM analysis, and Dr. Yael Feinstein-Rotkopf at the Core Research Facility of The Hadassah Medical School for managing the Nikon Yokogawa W1 Spinning Disk microscope. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

N2 - Background: Streptococcus mutans (S. mutans) plays a major role in the formation of dental caries. The aim of this study was to examine the effect of the green tea polyphenol, epigallocatechin gallate (EGCG), on biofilm formation of S. mutans. Methods: Following exposure to increasing concentrations of EGCG, the planktonic growth was measured by optical density and the biofilm biomass was quantified by crystal violet staining. Exopolysaccharides (EPS) production was visualized by confocal scanning laser microscopy, and the bacterial DNA content was determined by quantitative polymerase chain reaction (qPCR). Gene expression of selected genes was analyzed by real time (RT)-qPCR and membrane potential was examined by flow cytometry. Results: We observed that EGCG inhibited in a dose-dependent manner both the planktonic growth and the biofilm formation of S. mutans. Significant reduction of S. mutans biofilm formation, DNA content, and EPS production was observed at 2.2–4.4 mg/ml EGCG. EGCG reduced the expression of gtfB, gtfC and ftf genes involved in EPS production, and the nox and sodA genes involved in the protection against oxidative stress. Moreover, EGCG caused an immediate change in membrane potential. Conclusions: EGCG, a natural polyphenol, has a significant inhibitory effect on S. mutans dental biofilm formation and EPS production, and thus might be a potential drug in preventing dental caries.

AB - Background: Streptococcus mutans (S. mutans) plays a major role in the formation of dental caries. The aim of this study was to examine the effect of the green tea polyphenol, epigallocatechin gallate (EGCG), on biofilm formation of S. mutans. Methods: Following exposure to increasing concentrations of EGCG, the planktonic growth was measured by optical density and the biofilm biomass was quantified by crystal violet staining. Exopolysaccharides (EPS) production was visualized by confocal scanning laser microscopy, and the bacterial DNA content was determined by quantitative polymerase chain reaction (qPCR). Gene expression of selected genes was analyzed by real time (RT)-qPCR and membrane potential was examined by flow cytometry. Results: We observed that EGCG inhibited in a dose-dependent manner both the planktonic growth and the biofilm formation of S. mutans. Significant reduction of S. mutans biofilm formation, DNA content, and EPS production was observed at 2.2–4.4 mg/ml EGCG. EGCG reduced the expression of gtfB, gtfC and ftf genes involved in EPS production, and the nox and sodA genes involved in the protection against oxidative stress. Moreover, EGCG caused an immediate change in membrane potential. Conclusions: EGCG, a natural polyphenol, has a significant inhibitory effect on S. mutans dental biofilm formation and EPS production, and thus might be a potential drug in preventing dental caries.

KW - Caries

KW - Green-tea

KW - Oral bacteria

KW - Polyphenols

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SN - 1472-6831

VL - 21

JO - BMC Oral Health

JF - BMC Oral Health

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M1 - 447

ER -

Effect of epigallocatechin gallate on dental biofilm of Streptococcus mutans: An in vitro study

Abstract

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