Effect of mycoplasma on polyamine synthesis and levels in NIH 3T3 cells and in cells transformed by Rous sarcoma virus

Rajbabu Pakala; Reuven Laskov; Shlomo Rottem; Uriel Bachrach

doi:10.1111/j.1574-6968.1988.tb02757.x

Effect of mycoplasma on polyamine synthesis and levels in NIH 3T3 cells and in cells transformed by Rous sarcoma virus

Rajbabu Pakala, Reuven Laskov, Shlomo Rottem, Uriel Bachrach^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

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Abstract

Infecting NIH 3T3 cells with different species of mycoplasmas resulted only in a slight decrease in ornithine decarboxylase (ODC) activity and in the appearance of cadaverine in the infected cells. Similarly, the presence of mycoplasma in NIH 3T3 cells infected with a temperature-sensitive mutant of Rous Sarcoma virus did not bring about any significant changes either in the pattern of ODC activity or in putrescine levels, when transferred to the permissive temperature. This indicates that mycoplasmal contamination of cultures may not significantly change the putrescine metabolism in host cells. On the other hand, the presence of cadaverine in cultured cells may be attributed to contamination by mycoplasma.

Original language	English
Pages (from-to)	357-361
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	49
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1988.tb02757.x
State	Published - Mar 1988

Keywords

Mycoplasma
Ornithine decarboxylase
Polyamines

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10.1111/j.1574-6968.1988.tb02757.x

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title = "Effect of mycoplasma on polyamine synthesis and levels in NIH 3T3 cells and in cells transformed by Rous sarcoma virus",

abstract = "Infecting NIH 3T3 cells with different species of mycoplasmas resulted only in a slight decrease in ornithine decarboxylase (ODC) activity and in the appearance of cadaverine in the infected cells. Similarly, the presence of mycoplasma in NIH 3T3 cells infected with a temperature-sensitive mutant of Rous Sarcoma virus did not bring about any significant changes either in the pattern of ODC activity or in putrescine levels, when transferred to the permissive temperature. This indicates that mycoplasmal contamination of cultures may not significantly change the putrescine metabolism in host cells. On the other hand, the presence of cadaverine in cultured cells may be attributed to contamination by mycoplasma.",

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AU - Pakala, Rajbabu

AU - Laskov, Reuven

AU - Rottem, Shlomo

AU - Bachrach, Uriel

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AB - Infecting NIH 3T3 cells with different species of mycoplasmas resulted only in a slight decrease in ornithine decarboxylase (ODC) activity and in the appearance of cadaverine in the infected cells. Similarly, the presence of mycoplasma in NIH 3T3 cells infected with a temperature-sensitive mutant of Rous Sarcoma virus did not bring about any significant changes either in the pattern of ODC activity or in putrescine levels, when transferred to the permissive temperature. This indicates that mycoplasmal contamination of cultures may not significantly change the putrescine metabolism in host cells. On the other hand, the presence of cadaverine in cultured cells may be attributed to contamination by mycoplasma.

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Effect of mycoplasma on polyamine synthesis and levels in NIH 3T3 cells and in cells transformed by Rous sarcoma virus

Abstract

Keywords

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