Effect of oxazaborolidines on immobilized fructosyltransferase analyzed by surface plasmon resonance

Adel Jabbour, Moshe Shemesh, Morris Srebnik, Batia Zaks, Doron Steinberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Dental diseases are among the most prevalent afflictions of humankind. These diseases are associated with the formation of biofilms harboring pathogenic bacteria. Fructosyltransferases (FTF) are extra cellular enzymes of several oral bacteria. FTF are associated with the formation of extracellular polysaccharide matrix (fructans) which play a role in biofilm formation and oral bacteria physiology. Oxazaborolidines have been shown to inhibit biofilm formation. The purpose of this study was to examine if the anti-biofilm effect is, in part, an effect on the immobilized enzymes synthesizing the extra cellular polysaccharide participating in biofilm formation. Eight different oxazaborolidines (BNO1-BNO8) were synthesized and evaluated for their affect on the synthesis of fructans by FTF using the biomolecular interaction analysis (BIAcore) system which involves the use of real-time surface plasmon resonance (SPR) technique. The tested oxazaborolidines demonstrated a significant and immediate inhibitory effect on immobilized FTF activity. This effect was reversible. Our results show that oxazaborolidines can act as enzymatic inhibitors of FTF immobilized on the surface, also at levels lower than their MIC. Part of the anti-biofilm effect of BNOs may be accounted for this enzymatic inhibition.

Original languageAmerican English
Pages (from-to)1658-1663
Number of pages6
JournalBiosensors and Bioelectronics
Issue number8
StatePublished - 15 Mar 2007

Bibliographical note

Funding Information:
This work was supported in part by the Israel Science Foundation. This study is part of the Ph.D. dissertation of Moshe Shemesh and Adel Jabbour.


  • Fructosyltransferase
  • Immobilized enzyme
  • Oral bacteria
  • Oxazaborolidines


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