TY - JOUR
T1 - Effect of substance P on Rat gastrointestinal transit
AU - Silkoff, P.
AU - Karmeli, F.
AU - Goldin, E.
AU - Ewenson, A.
AU - Gilon, C.
AU - Chorev, M.
AU - Laufer, R.
AU - Selinger, Z.
AU - Rachmilewitz, D.
PY - 1988/1
Y1 - 1988/1
N2 - The in vivo effect of substance P and related peptide analogs on gastrointestinal transit in unanesthetized rats was studied. Fasted male rats were given intragastrically 0.5 ml of a powdered charcoal (BaSo4·H2O) meal and were concomitantly injected intraperitoneally with 8 Μg/kg of substance P or a related peptide. In control rats, the percentage of small intestine traversed by the meal 15 min after feeding was 44.9±1.4 (N = 12). Substance P, [Glu6]SP6-11, [pGlu6, gPhe6, mGly9]SP6-11 and [pGlu5, N-MePhe8, N-MeGly9SP6-11, significantly accelerated intestinal transit: 59.5±3.1% (N=7); 66.0±3.8% (N=14), 66.8±2.4% (N=25), and 58.4±4.4% (N=4), respectively. Concomitant injection of [pGlu6SP6-11 and BOC-Phe-Phe-Gly-NHOH, an inhibitor of enzyme degradation at a dose of 800 Μg/kg lowered by 10-fold the dose of [pGlu6]SP6-11 needed to induce the same degree of intestinal transit acceleration. These results indicate that in rats, substance P and related peptides accelerate gastrointestinal transit.
AB - The in vivo effect of substance P and related peptide analogs on gastrointestinal transit in unanesthetized rats was studied. Fasted male rats were given intragastrically 0.5 ml of a powdered charcoal (BaSo4·H2O) meal and were concomitantly injected intraperitoneally with 8 Μg/kg of substance P or a related peptide. In control rats, the percentage of small intestine traversed by the meal 15 min after feeding was 44.9±1.4 (N = 12). Substance P, [Glu6]SP6-11, [pGlu6, gPhe6, mGly9]SP6-11 and [pGlu5, N-MePhe8, N-MeGly9SP6-11, significantly accelerated intestinal transit: 59.5±3.1% (N=7); 66.0±3.8% (N=14), 66.8±2.4% (N=25), and 58.4±4.4% (N=4), respectively. Concomitant injection of [pGlu6SP6-11 and BOC-Phe-Phe-Gly-NHOH, an inhibitor of enzyme degradation at a dose of 800 Μg/kg lowered by 10-fold the dose of [pGlu6]SP6-11 needed to induce the same degree of intestinal transit acceleration. These results indicate that in rats, substance P and related peptides accelerate gastrointestinal transit.
KW - gastrointestinal transit in rats
KW - substance P
UR - http://www.scopus.com/inward/record.url?scp=0023891970&partnerID=8YFLogxK
U2 - 10.1007/BF01536634
DO - 10.1007/BF01536634
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C2 - 2448096
AN - SCOPUS:0023891970
SN - 0163-2116
VL - 33
SP - 74
EP - 77
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 1
ER -