Effect of substance P on Rat gastrointestinal transit

P. Silkoff; F. Karmeli; E. Goldin; A. Ewenson; C. Gilon; M. Chorev; R. Laufer; Z. Selinger; D. Rachmilewitz

doi:10.1007/BF01536634

Effect of substance P on Rat gastrointestinal transit

P. Silkoff
, F. Karmeli
, E. Goldin
, A. Ewenson
, C. Gilon
, M. Chorev
, R. Laufer
, Z. Selinger
, D. Rachmilewitz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The in vivo effect of substance P and related peptide analogs on gastrointestinal transit in unanesthetized rats was studied. Fasted male rats were given intragastrically 0.5 ml of a powdered charcoal (BaSo₄·H₂O) meal and were concomitantly injected intraperitoneally with 8 Μg/kg of substance P or a related peptide. In control rats, the percentage of small intestine traversed by the meal 15 min after feeding was 44.9±1.4 (N = 12). Substance P, [Glu⁶]SP_6-11, [pGlu⁶, gPhe⁶, mGly⁹]SP_6-11 and [pGlu⁵, N-MePhe⁸, N-MeGly⁹SP_6-11, significantly accelerated intestinal transit: 59.5±3.1% (N=7); 66.0±3.8% (N=14), 66.8±2.4% (N=25), and 58.4±4.4% (N=4), respectively. Concomitant injection of [pGlu⁶SP_6-11 and BOC-Phe-Phe-Gly-NHOH, an inhibitor of enzyme degradation at a dose of 800 Μg/kg lowered by 10-fold the dose of [pGlu⁶]SP_6-11 needed to induce the same degree of intestinal transit acceleration. These results indicate that in rats, substance P and related peptides accelerate gastrointestinal transit.

Original language	English
Pages (from-to)	74-77
Number of pages	4
Journal	Digestive Diseases and Sciences
Volume	33
Issue number	1
DOIs	https://doi.org/10.1007/BF01536634
State	Published - Jan 1988

Keywords

gastrointestinal transit in rats
substance P

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10.1007/BF01536634

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title = "Effect of substance P on Rat gastrointestinal transit",

abstract = "The in vivo effect of substance P and related peptide analogs on gastrointestinal transit in unanesthetized rats was studied. Fasted male rats were given intragastrically 0.5 ml of a powdered charcoal (BaSo4·H2O) meal and were concomitantly injected intraperitoneally with 8 Μg/kg of substance P or a related peptide. In control rats, the percentage of small intestine traversed by the meal 15 min after feeding was 44.9±1.4 (N = 12). Substance P, [Glu6]SP6-11, [pGlu6, gPhe6, mGly9]SP6-11 and [pGlu5, N-MePhe8, N-MeGly9SP6-11, significantly accelerated intestinal transit: 59.5±3.1\% (N=7); 66.0±3.8\% (N=14), 66.8±2.4\% (N=25), and 58.4±4.4\% (N=4), respectively. Concomitant injection of [pGlu6SP6-11 and BOC-Phe-Phe-Gly-NHOH, an inhibitor of enzyme degradation at a dose of 800 Μg/kg lowered by 10-fold the dose of [pGlu6]SP6-11 needed to induce the same degree of intestinal transit acceleration. These results indicate that in rats, substance P and related peptides accelerate gastrointestinal transit.",

keywords = "gastrointestinal transit in rats, substance P",

author = "P. Silkoff and F. Karmeli and E. Goldin and A. Ewenson and C. Gilon and M. Chorev and R. Laufer and Z. Selinger and D. Rachmilewitz",

year = "1988",

month = jan,

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AU - Silkoff, P.

AU - Karmeli, F.

AU - Goldin, E.

AU - Ewenson, A.

AU - Gilon, C.

AU - Chorev, M.

AU - Laufer, R.

AU - Selinger, Z.

AU - Rachmilewitz, D.

PY - 1988/1

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N2 - The in vivo effect of substance P and related peptide analogs on gastrointestinal transit in unanesthetized rats was studied. Fasted male rats were given intragastrically 0.5 ml of a powdered charcoal (BaSo4·H2O) meal and were concomitantly injected intraperitoneally with 8 Μg/kg of substance P or a related peptide. In control rats, the percentage of small intestine traversed by the meal 15 min after feeding was 44.9±1.4 (N = 12). Substance P, [Glu6]SP6-11, [pGlu6, gPhe6, mGly9]SP6-11 and [pGlu5, N-MePhe8, N-MeGly9SP6-11, significantly accelerated intestinal transit: 59.5±3.1% (N=7); 66.0±3.8% (N=14), 66.8±2.4% (N=25), and 58.4±4.4% (N=4), respectively. Concomitant injection of [pGlu6SP6-11 and BOC-Phe-Phe-Gly-NHOH, an inhibitor of enzyme degradation at a dose of 800 Μg/kg lowered by 10-fold the dose of [pGlu6]SP6-11 needed to induce the same degree of intestinal transit acceleration. These results indicate that in rats, substance P and related peptides accelerate gastrointestinal transit.

AB - The in vivo effect of substance P and related peptide analogs on gastrointestinal transit in unanesthetized rats was studied. Fasted male rats were given intragastrically 0.5 ml of a powdered charcoal (BaSo4·H2O) meal and were concomitantly injected intraperitoneally with 8 Μg/kg of substance P or a related peptide. In control rats, the percentage of small intestine traversed by the meal 15 min after feeding was 44.9±1.4 (N = 12). Substance P, [Glu6]SP6-11, [pGlu6, gPhe6, mGly9]SP6-11 and [pGlu5, N-MePhe8, N-MeGly9SP6-11, significantly accelerated intestinal transit: 59.5±3.1% (N=7); 66.0±3.8% (N=14), 66.8±2.4% (N=25), and 58.4±4.4% (N=4), respectively. Concomitant injection of [pGlu6SP6-11 and BOC-Phe-Phe-Gly-NHOH, an inhibitor of enzyme degradation at a dose of 800 Μg/kg lowered by 10-fold the dose of [pGlu6]SP6-11 needed to induce the same degree of intestinal transit acceleration. These results indicate that in rats, substance P and related peptides accelerate gastrointestinal transit.

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Effect of substance P on Rat gastrointestinal transit

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