TY - JOUR
T1 - Effect of temperature on the interactions between dipolar membranes
AU - Szekely, Pablo
AU - Asor, Roi
AU - Dvir, Tom
AU - Szekely, Or
AU - Raviv, Uri
PY - 2012/3/22
Y1 - 2012/3/22
N2 - It is well-known that phospholipids in aqueous environment self-assemble into lamellar structures with a repeat distance governed by the interactions between them. Yet, the understanding of these interactions is incomplete. In this paper, we study the effect of temperature on the interlamellar interactions between dipolar membranes. Using solution small-angle X-ray scattering (SAXS), we measured the repeat distance between 1,2-dilauroyl-sn-glycero-3- phosphocholine (DLPC) bilayers at different temperatures and osmotic stresses. We found that when no pressure is applied the lamellar repeat distance, D, decreases and then increases with increasing temperature. As the osmotic stress increases, D decreases with temperature and then increases to a limited extent, until at sufficiently high pressure D decreases with temperature in all the examined range. We then reconstructed experimentally the equation of state and fit it with a modified interaction model that takes into account the temperature dependence of the fluctuation term. Finally, we showed how the thickness of DLPC membranes decreases with temperature.
AB - It is well-known that phospholipids in aqueous environment self-assemble into lamellar structures with a repeat distance governed by the interactions between them. Yet, the understanding of these interactions is incomplete. In this paper, we study the effect of temperature on the interlamellar interactions between dipolar membranes. Using solution small-angle X-ray scattering (SAXS), we measured the repeat distance between 1,2-dilauroyl-sn-glycero-3- phosphocholine (DLPC) bilayers at different temperatures and osmotic stresses. We found that when no pressure is applied the lamellar repeat distance, D, decreases and then increases with increasing temperature. As the osmotic stress increases, D decreases with temperature and then increases to a limited extent, until at sufficiently high pressure D decreases with temperature in all the examined range. We then reconstructed experimentally the equation of state and fit it with a modified interaction model that takes into account the temperature dependence of the fluctuation term. Finally, we showed how the thickness of DLPC membranes decreases with temperature.
UR - http://www.scopus.com/inward/record.url?scp=84858992650&partnerID=8YFLogxK
U2 - 10.1021/jp209157y
DO - 10.1021/jp209157y
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:84858992650
SN - 1520-6106
VL - 116
SP - 3519
EP - 3524
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 11
ER -