Effect of Triton X-100 on the Hydrolysis of Sphingomyelin by Sphingomyelinase of Rat Brain

Saul Yedgar, Shimon Gatt

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Abstract

Mixed dispersions of the nonionic detergent Triton X-100 and sphingomyelin were used as substrate for sphingomyelinase of rat brain. The dependence of the rate of hydrolysis on the concentration of sphingomyelin was measured in two ways: at a fixed concentration of Triton X-100 or at varying concentrations of this detergent, while maintaining a fixed molar ratio of Triton X-100 to sphingomyelin. In either case, the v vs. S curves deviated from the hyperbolic shape predicted by the Michaelis-Menten kinetic theory. These deviations are discussed and interpreted on the basis of the physicochemical properties of the mixed dispersions of detergent and lipid studied in previous papers.

Original languageEnglish
Pages (from-to)2570-2573
Number of pages4
JournalBiochemistry
Volume15
Issue number12
DOIs
StatePublished - 1 Jun 1976

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