Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells

Kamal Dabbah, Ilana Perelshtein, Aharon Gedanken, Yael Houri-Haddad, Osnat Feuerstein*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    2 Scopus citations


    This study aims to investigate the effects of a novel ZnCuO nanoparticle coating for dental implants—versus those of conventional titanium surfaces—on bacteria and host cells. A multispecies biofilm composed of Streptococcus sanguinis, Actinomyces naeslundii, Porphyromonas gingivalis, and Fusobacterium nucleatum was grown for 14 days on various titanium discs: machined, sandblasted, sandblasted and acid-etched (SLA), ZnCuO-coated, and hydroxyapatite discs. Bacterial species were quantified with qPCR, and their viability was examined via confocal microscopy. Osteoblast-like and macrophage-like cells grown on the various discs for 48 h were examined for proliferation using an XTT assay, and for activity using ALP and TNF-α assays. The CSLM revealed more dead bacteria in biofilms grown on titanium than on hydroxyapatite, and less on sandblasted than on machined and ZnCuO-coated surfaces, with the latter showing a significant decrease in all four biofilm species. The osteoblast-like cells showed increased proliferation on all of the titanium surfaces, with higher activity on the ZnCuO-coated and sandblasted discs. The macrophage-like cells showed higher proliferation on the hydroxyapatite and sandblasted discs, and lower activity on the SLA and ZnCuO-coated discs. The ZnCuO-coated titanium has anti-biofilm characteristics with desired effects on host cells, thus representing a promising candidate in the complex battle against peri-implantitis.

    Original languageAmerican English
    Article number2514
    Issue number7
    StatePublished - 29 Mar 2022

    Bibliographical note

    Publisher Copyright:
    © 2022 by the authors. Licensee MDPI, Basel, Switzerland.


    • antimicrobials/antimicrobial resistance
    • biofilm model
    • biofilm(s)
    • dental implant(s)
    • immunity
    • macrophage(s)
    • osteoblast(s)
    • peri-implant infection(s)
    • zinc oxide


    Dive into the research topics of 'Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells'. Together they form a unique fingerprint.

    Cite this