TY - JOUR
T1 - Effects of ATP or phosphate on passive rubidium fluxes mediated by Na‐K‐ATPase reconstituted into phospholipid vesicles
AU - Karlish, S. J.D.
AU - Stein, W. D.
PY - 1982/7/1
Y1 - 1982/7/1
N2 - 1. The passive Rb fluxes mediated by the Na—K pump in reconstituted vesicles, described by Karlish & Stein (1982), are affected by ATP or by phosphate acting separately. 2. Rb—Rb exchange through inside‐out pumps is stimulated by ATP at low concentrations and is inhibited at high concentrations. There are mutual effects of Rb at cytoplasmic sites and ATP. The higher is the Rb concentration, the greater is the degree of stimulation and the less is the inhibition of exchange by ATP, and the higher are the concentrations of ATP required to produce effects. ATP stimulates Rb—Rb exchange maximally by about 5‐fold. 3. There are similar effects of ATP on zero‐trans net Rb uptake through inside‐out pumps. However, much lower degrees of stimulation and greater inhibition of the net flux by ATP are observed, and much lower concentrations of ATP are required for these effects, by comparison with those on Rb—Rb exchange. 4. Rb uptake on inside‐out pumps in Na‐loaded vesicles shows only inhibition by ATP. 5. Phosphate effects require the presence of Mg0 ions. At low Mg0 concentrations (up to 100 μM) phosphate moderately stimulates Rb uptake into Rb‐free or Rb‐loaded vesicles (about 50%), but has no effect on Rb uptake into Na‐loaded vesicles. At millimolar concentrations of Mg0 ions, phosphate strongly inhibits the Rb uptake into Rb‐free or Na‐loaded vesicles but has no effect on Rb uptake into Rb‐loaded vesicles. 6. The separate effects of ATP and of phosphate are explained in terms of the model proposed by Karlish & Stein (1982), modified to take into account stimulation of the conformational transition E2(Rb)occ → E1 Rb by ATP, and stimulation of the conformational transition E2(Rb)occ → E2 Rb by phosphate due to phosphorylation of the protein.
AB - 1. The passive Rb fluxes mediated by the Na—K pump in reconstituted vesicles, described by Karlish & Stein (1982), are affected by ATP or by phosphate acting separately. 2. Rb—Rb exchange through inside‐out pumps is stimulated by ATP at low concentrations and is inhibited at high concentrations. There are mutual effects of Rb at cytoplasmic sites and ATP. The higher is the Rb concentration, the greater is the degree of stimulation and the less is the inhibition of exchange by ATP, and the higher are the concentrations of ATP required to produce effects. ATP stimulates Rb—Rb exchange maximally by about 5‐fold. 3. There are similar effects of ATP on zero‐trans net Rb uptake through inside‐out pumps. However, much lower degrees of stimulation and greater inhibition of the net flux by ATP are observed, and much lower concentrations of ATP are required for these effects, by comparison with those on Rb—Rb exchange. 4. Rb uptake on inside‐out pumps in Na‐loaded vesicles shows only inhibition by ATP. 5. Phosphate effects require the presence of Mg0 ions. At low Mg0 concentrations (up to 100 μM) phosphate moderately stimulates Rb uptake into Rb‐free or Rb‐loaded vesicles (about 50%), but has no effect on Rb uptake into Na‐loaded vesicles. At millimolar concentrations of Mg0 ions, phosphate strongly inhibits the Rb uptake into Rb‐free or Na‐loaded vesicles but has no effect on Rb uptake into Rb‐loaded vesicles. 6. The separate effects of ATP and of phosphate are explained in terms of the model proposed by Karlish & Stein (1982), modified to take into account stimulation of the conformational transition E2(Rb)occ → E1 Rb by ATP, and stimulation of the conformational transition E2(Rb)occ → E2 Rb by phosphate due to phosphorylation of the protein.
UR - http://www.scopus.com/inward/record.url?scp=0020001046&partnerID=8YFLogxK
U2 - 10.1113/jphysiol.1982.sp014266
DO - 10.1113/jphysiol.1982.sp014266
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C2 - 6290647
AN - SCOPUS:0020001046
SN - 0022-3751
VL - 328
SP - 317
EP - 331
JO - Journal of Physiology
JF - Journal of Physiology
IS - 1
ER -