Effects of intra-axonal injection of Ca2+ buffers on evoked release and on facilitation in the crayfish neuromuscular junction

Binyamin Hochner, Hanna Parnas, Itzchak Parnas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Ca2+ buffers were injected into the excitatory axon of the crayfish opener muscle. The magnitude and time course of evoked release and of facilitation were measured. EGTA (on-rate about 106 M-1s-1 had no effect on evoked release but reduced facilitation. BAPTA and nitr-5, buffers with similar Kd's but faster on-rates, reduced both evoked release and facilitation. However, these buffers had no effect on the time course of evoked release. These results show that fast Ca2+ buffers reduce the Ca2+ transient associated with evoked release and also the level of residual Ca2+ involved in facilitation. However, Ca2+ buffering is not the mechanism which controls the time course of release.

Original languageEnglish
Pages (from-to)215-218
Number of pages4
JournalNeuroscience Letters
Volume125
Issue number2
DOIs
StatePublished - 29 Apr 1991

Keywords

  • Ca buffer
  • Facilitation
  • Neuromuscular junction
  • Synapse
  • Transmitter release

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