Abstract
Ca2+ buffers were injected into the excitatory axon of the crayfish opener muscle. The magnitude and time course of evoked release and of facilitation were measured. EGTA (on-rate about 106 M-1s-1 had no effect on evoked release but reduced facilitation. BAPTA and nitr-5, buffers with similar Kd's but faster on-rates, reduced both evoked release and facilitation. However, these buffers had no effect on the time course of evoked release. These results show that fast Ca2+ buffers reduce the Ca2+ transient associated with evoked release and also the level of residual Ca2+ involved in facilitation. However, Ca2+ buffering is not the mechanism which controls the time course of release.
Original language | English |
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Pages (from-to) | 215-218 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 125 |
Issue number | 2 |
DOIs | |
State | Published - 29 Apr 1991 |
Keywords
- Ca buffer
- Facilitation
- Neuromuscular junction
- Synapse
- Transmitter release