Effects of mono(2-ethylhexyl)phthalate on cytoplasmic maturation of oocytes - The bovine model

D. Kalo, Z. Roth*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Phthalates are known reproductive toxicants, but their intracellular disruptive effects on oocyte maturation competence are less known. We studied the potential risk associated with acute exposure of oocytes to mono(2-ethylhexyl)phthalate (MEHP). First, bovine oocytes were matured in vitro with or without 50. μM MEHP and examined for mitochondrial features associated with DNA fragmentation. MEHP increased reactive oxygen species levels and reduced the proportion of highly polarized mitochondria along with alterations in genes associated with mitochondrial oxidative phosphorylation (. CYC1, MT-CO1 and ATP5B). In a second set of experiments, we associated the effects of MEHP on meiotic progression with those on cytoplasmic maturation. MEHP impaired reorganization of cytoplasmic organelles in matured oocytes reflected by reductions in category I mitochondria, type III cortical granules and class I endoplasmic reticulum. These alterations are associated with the previously reported reduced developmental competence of MEHP-treated bovine oocytes, and reveal the risk associated with acute exposure.

Original languageAmerican English
Pages (from-to)141-151
Number of pages11
JournalReproductive Toxicology
StatePublished - 1 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.


  • Bovine
  • Cytoplasmic maturation
  • Oocyte
  • Phthalate


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