Effects of testosterone and 17, β - estradiol on the polyamine metabolism in cultivated normal rat kidney epithelial cells

I. Jotova*, C. Wang, A. Tabib, O. Dimitrov, U. Bachrach

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Ornithine decarboxylase (ODC) and diamine oxidase (DAO) are important enzymes involved in the metabolism of polyamines (putrescine, spermidine and spermine). The influence of testosterone (T) and 17, β estradiol (E2) on the activity of ODC and DAO was examined in cultivated normal rat kidney (NRK) epithelial cells. The results showed an increase in enzyme activities 4 hours or 12 hours after hormonal treatment. Both T and E2 led to a significant increase (1.6-fold) in ODC protein level as compared to the controls. Cellular concentration of spermidine and spermine increased (2.2- and 2.6-fold respectively) 4 hours after T addition. A higher levels in concentrations of putrescine (1.4-fold) and spermine (1.5-fold) 12 hours after E2 treatment were observed. These results suggest that the biosynthesis and terminal oxidation of the polyamines in NRK epithelial cells are androgenand estrogen-mediated and depend on the hormonal sensitivity of the cells.

Original languageAmerican English
Pages (from-to)353-361
Number of pages9
JournalAmino Acids
Volume18
Issue number4
DOIs
StatePublished - 2000

Keywords

  • 17 β - estradiol
  • Amino acids
  • Diamine oxidase
  • Ornithine decarboxylase
  • Polyamines
  • Testosterone

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