Abstract
Ornithine decarboxylase (ODC) and diamine oxidase (DAO) are important enzymes involved in the metabolism of polyamines (putrescine, spermidine and spermine). The influence of testosterone (T) and 17, β estradiol (E2) on the activity of ODC and DAO was examined in cultivated normal rat kidney (NRK) epithelial cells. The results showed an increase in enzyme activities 4 hours or 12 hours after hormonal treatment. Both T and E2 led to a significant increase (1.6-fold) in ODC protein level as compared to the controls. Cellular concentration of spermidine and spermine increased (2.2- and 2.6-fold respectively) 4 hours after T addition. A higher levels in concentrations of putrescine (1.4-fold) and spermine (1.5-fold) 12 hours after E2 treatment were observed. These results suggest that the biosynthesis and terminal oxidation of the polyamines in NRK epithelial cells are androgenand estrogen-mediated and depend on the hormonal sensitivity of the cells.
Original language | English |
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Pages (from-to) | 353-361 |
Number of pages | 9 |
Journal | Amino Acids |
Volume | 18 |
Issue number | 4 |
DOIs | |
State | Published - 2000 |
Keywords
- 17 β - estradiol
- Amino acids
- Diamine oxidase
- Ornithine decarboxylase
- Polyamines
- Testosterone