Effects of uranyl ions on lipid bilayer membranes

Hagai Ginsburg; J. Mario Wolosin

doi:10.1016/0009-3084(79)90040-9

Effects of uranyl ions on lipid bilayer membranes

Hagai Ginsburg^*
, J. Mario Wolosin

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Uranyl ions (UO₂²⁺) stabilize black lipid membranes (BLM's) as inferred from the doubling of the breakdown voltage and from a considerable increase in the lifetime of the BLM's. These effects are observed also in BLM's made of mono-olein and of oxidized cholesterol. The lytic effect of lysolecithin is significantly reduced in the presence of UO₂²⁺. Uranyl ions adsorb to the interface of BLM's made of phosphatidylcholine (PC) with a dissociation constant of about 3 : 10^-6 M and thereby charge the interface of the membrane and attain almost stoichiometric binding of one molecule of uranyl ion per one molecule of PC at 1 M ionic strength and 20 μM of UO₂²⁺. The membrane conductance induced by ionophores is considerably reduced by UO₂²⁺ and it is inferred by various tests that this is due to the charging of the interface and not to changes in membrane fluidity.

Original language	English
Pages (from-to)	125-131
Number of pages	7
Journal	Chemistry and Physics of Lipids
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0009-3084(79)90040-9
State	Published - 1979

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title = "Effects of uranyl ions on lipid bilayer membranes",

abstract = "Uranyl ions (UO22+) stabilize black lipid membranes (BLM's) as inferred from the doubling of the breakdown voltage and from a considerable increase in the lifetime of the BLM's. These effects are observed also in BLM's made of mono-olein and of oxidized cholesterol. The lytic effect of lysolecithin is significantly reduced in the presence of UO22+. Uranyl ions adsorb to the interface of BLM's made of phosphatidylcholine (PC) with a dissociation constant of about 3 : 10-6 M and thereby charge the interface of the membrane and attain almost stoichiometric binding of one molecule of uranyl ion per one molecule of PC at 1 M ionic strength and 20 μM of UO22+. The membrane conductance induced by ionophores is considerably reduced by UO22+ and it is inferred by various tests that this is due to the charging of the interface and not to changes in membrane fluidity.",

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AU - Ginsburg, Hagai

AU - Wolosin, J. Mario

PY - 1979

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N2 - Uranyl ions (UO22+) stabilize black lipid membranes (BLM's) as inferred from the doubling of the breakdown voltage and from a considerable increase in the lifetime of the BLM's. These effects are observed also in BLM's made of mono-olein and of oxidized cholesterol. The lytic effect of lysolecithin is significantly reduced in the presence of UO22+. Uranyl ions adsorb to the interface of BLM's made of phosphatidylcholine (PC) with a dissociation constant of about 3 : 10-6 M and thereby charge the interface of the membrane and attain almost stoichiometric binding of one molecule of uranyl ion per one molecule of PC at 1 M ionic strength and 20 μM of UO22+. The membrane conductance induced by ionophores is considerably reduced by UO22+ and it is inferred by various tests that this is due to the charging of the interface and not to changes in membrane fluidity.

AB - Uranyl ions (UO22+) stabilize black lipid membranes (BLM's) as inferred from the doubling of the breakdown voltage and from a considerable increase in the lifetime of the BLM's. These effects are observed also in BLM's made of mono-olein and of oxidized cholesterol. The lytic effect of lysolecithin is significantly reduced in the presence of UO22+. Uranyl ions adsorb to the interface of BLM's made of phosphatidylcholine (PC) with a dissociation constant of about 3 : 10-6 M and thereby charge the interface of the membrane and attain almost stoichiometric binding of one molecule of uranyl ion per one molecule of PC at 1 M ionic strength and 20 μM of UO22+. The membrane conductance induced by ionophores is considerably reduced by UO22+ and it is inferred by various tests that this is due to the charging of the interface and not to changes in membrane fluidity.

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JO - Chemistry and Physics of Lipids

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Effects of uranyl ions on lipid bilayer membranes

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