Efficacy evaluation of a novel submicron amphotericin b emulsion in murine candidiasis

A submicron amphotericin B (AmB) sterile emulsion with a mean droplet size of approximately 100 nm was prepared. The emulsion was stable at 4°C over a period of 4 months. The acute toxicity results showed that the maximum tolerated dose of the AmB emulsion was 2·0 mg kg^-1 as compared to 0·5 mg kg^-1 for a commercial deoxycholate suspension, Fungizone® Efficacy evaluations of submicron AmB emulsion compared to Fungizone® were performed in a murine candidiasis model using either a single or a multiple daily dose administration of 0·4 mg kg^-1. Survival (100%) was observed up to 15-18 days post-infection in mice treated with AmB emulsion or Fungizone® while all control non-treated animals had died after 9 days in both single and multi-treatment experiments. Survival (50%) was obtained after 35 days in the single treatment, and 40 days in the multi-treatment, experiment with AmB emulsion, while treatment with Fungizone® gave 50% survival after 15 and 20 days, respectively. The overall results indicate that in murine candidiasis, treatment with AmB submicron emulsion was more effective than Fungizone® suggesting a potential therapeutic application.