Efficient generation of viral and integration-free human induced pluripotent stem cell-derived oligodendrocytes

Araceli Espinosa-Jeffrey; Bruno Blanchi; Juan Carlos Biancotti; Shalini Kumar; Megumi Hirose; Berhan Mandefro; Dodanim Talavera-Adame; Nissim Benvenisty; Jean de Vellis

doi:10.1002/cpsc.11

Efficient generation of viral and integration-free human induced pluripotent stem cell-derived oligodendrocytes

Araceli Espinosa-Jeffrey, Bruno Blanchi, Juan Carlos Biancotti, Shalini Kumar, Megumi Hirose, Berhan Mandefro, Dodanim Talavera-Adame, Nissim Benvenisty, Jean de Vellis

Department of Genetics

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral- and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling.

Original language	American English
Journal	Current Protocols in Stem Cell Biology
Volume	2016
DOIs	https://doi.org/10.1002/cpsc.11
State	Published - Aug 2016

Bibliographical note

Funding Information:
We thank Dr. W. Lowry for hiPS21 and 23. This work was supported in part by NIH HD06576 and by a Pilot Grant from the National Multiple Sclerosis Society PP1498 (de Vellis/Espinosa-Jeffrey).

Publisher Copyright:
© 2016 by John Wiley & Sons, Inc.

Keywords

Chemically defined media
Human induced pluripotent stem cells
Lineage progression
NSC
Neural stem cells
Neurospheres
Oligodendrocyte maturation
Oligodendrocyte specification
Oligospheres

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/cpsc.11

Cite this

@article{d2eeca37da98470f8140802e438b18a4,

title = "Efficient generation of viral and integration-free human induced pluripotent stem cell-derived oligodendrocytes",

abstract = "Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral- and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling.",

keywords = "Chemically defined media, Human induced pluripotent stem cells, Lineage progression, NSC, Neural stem cells, Neurospheres, Oligodendrocyte maturation, Oligodendrocyte specification, Oligospheres",

author = "Araceli Espinosa-Jeffrey and Bruno Blanchi and Biancotti, {Juan Carlos} and Shalini Kumar and Megumi Hirose and Berhan Mandefro and Dodanim Talavera-Adame and Nissim Benvenisty and {de Vellis}, Jean",

note = "Funding Information: We thank Dr. W. Lowry for hiPS21 and 23. This work was supported in part by NIH HD06576 and by a Pilot Grant from the National Multiple Sclerosis Society PP1498 (de Vellis/Espinosa-Jeffrey). Publisher Copyright: {\textcopyright} 2016 by John Wiley & Sons, Inc.",

year = "2016",

month = aug,

doi = "10.1002/cpsc.11",

language = "American English",

volume = "2016",

journal = "Current Protocols in Stem Cell Biology",

issn = "1941-7322",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Efficient generation of viral and integration-free human induced pluripotent stem cell-derived oligodendrocytes

AU - Espinosa-Jeffrey, Araceli

AU - Blanchi, Bruno

AU - Biancotti, Juan Carlos

AU - Kumar, Shalini

AU - Hirose, Megumi

AU - Mandefro, Berhan

AU - Talavera-Adame, Dodanim

AU - Benvenisty, Nissim

AU - de Vellis, Jean

N1 - Funding Information: We thank Dr. W. Lowry for hiPS21 and 23. This work was supported in part by NIH HD06576 and by a Pilot Grant from the National Multiple Sclerosis Society PP1498 (de Vellis/Espinosa-Jeffrey). Publisher Copyright: © 2016 by John Wiley & Sons, Inc.

PY - 2016/8

Y1 - 2016/8

N2 - Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral- and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling.

AB - Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral- and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling.

KW - Chemically defined media

KW - Human induced pluripotent stem cells

KW - Lineage progression

KW - NSC

KW - Neural stem cells

KW - Neurospheres

KW - Oligodendrocyte maturation

KW - Oligodendrocyte specification

KW - Oligospheres

UR - http://www.scopus.com/inward/record.url?scp=84991637906&partnerID=8YFLogxK

U2 - 10.1002/cpsc.11

DO - 10.1002/cpsc.11

M3 - Article

C2 - 27532816

AN - SCOPUS:84991637906

SN - 1941-7322

VL - 2016

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

ER -

Efficient generation of viral and integration-free human induced pluripotent stem cell-derived oligodendrocytes

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this