TY - JOUR
T1 - Efficient generation of viral and integration-free human induced pluripotent stem cell-derived oligodendrocytes
AU - Espinosa-Jeffrey, Araceli
AU - Blanchi, Bruno
AU - Biancotti, Juan Carlos
AU - Kumar, Shalini
AU - Hirose, Megumi
AU - Mandefro, Berhan
AU - Talavera-Adame, Dodanim
AU - Benvenisty, Nissim
AU - De Vellis, Jean
N1 - Publisher Copyright:
© 2016 by John Wiley & Sons, Inc.
PY - 2016/11
Y1 - 2016/11
N2 - Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral-and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling.
AB - Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral-and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling.
KW - Chemically defined media
KW - Human induced pluripotent stem cells
KW - Lineage progression
KW - NSC
KW - Neural stem cells
KW - Neurospheres
KW - Oligodendrocyte maturation
KW - Oligodendrocyte specification
KW - Oligospheres
UR - http://www.scopus.com/inward/record.url?scp=85026643679&partnerID=8YFLogxK
U2 - 10.1002/cpsc.19
DO - 10.1002/cpsc.19
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AN - SCOPUS:85026643679
SN - 1941-7322
VL - 2016
SP - 1
EP - 28
JO - Current Protocols in Stem Cell Biology
JF - Current Protocols in Stem Cell Biology
ER -