Abstract
The S. cerevisiae CDC40 gene was originally identified as a cell-division-specific gene that is essential only at elevated temperatures. Cells carrying mutations in this gene arrest with a large bud and a single nucleus with duplicated DNA content. Cdc40p is also required for spindle establishment or maintenance. Sequence analysis reveals that CDC40 is identical to PRP17, a gene involved in pre-mRNA splicing. In this paper, we show that Cdc40p is required at all temperatures for efficient entry into S-phase and that cell cycle arrest associated with cdc40 mutations is independent of all the known checkpoint mechanisms. Using immunofluorescence, we show that Cdc40p is localized to the nuclear membrane, weakly associated with the nuclear pore. Our results point to a link between cell cycle progression, pre-mRNA splicing, and mRNA export.
| Original language | English |
|---|---|
| Pages (from-to) | 232-241 |
| Number of pages | 10 |
| Journal | Molecular and General Genetics |
| Volume | 260 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
Bibliographical note
Funding Information:Acknowledgments We thank G. Fink for many fruitful discussions. We thank B. Byers, S. Fields, G. Fink, B. Futcher, L. Gotch, E. Hurt, M. Johnston, S. Reed, A. Sugino, and A. Tzagaloff for plasmids and yeast strains. We thank C. Cole for antibodies. This research was supported by grants from the US-Israel Binational Science Foundation, Jerusalem, Israel to Y.K. Israel Cancer Association to M.K., and Israel Cancer Research fund to M.K.
Keywords
- Cell cycle
- Saccharomyces cerevisiae
- mRNA splicing