Ekspressiia gena khloramfenikolatsetiltransferazy pod kontrolem nekotorykh promotorov E. coli i bakteriofaga lambda.

S. V. Mashko; S. M. Podkovyrov; M. E. Trukhan; R. L. Gorovits; M. I. Lebedeva

Ekspressiia gena khloramfenikolatsetiltransferazy pod kontrolem nekotorykh promotorov E. coli i bakteriofaga lambda.

Translated title of the contribution: Expression of the chloramphenicol acetyltransferase gene is under control of various promoters of E. coli and phage lambda

S. V. Mashko^*, S. M. Podkovyrov, M. E. Trukhan, R. L. Gorovits, M. I. Lebedeva

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Plasmids have been constructed with the structural region of the cat gene being under the control of the lactose (lacUV5), tryptophane (trpOP), operons of Escherichia coli, the hybrid trp-lac (tac) promoter and early bacteriophage lambda promoters (PL, PR and PLIT). The expression of chloramphenicolacetyltransferase gene in Escherichia coli cells harbouring such recombinant plasmids and pBR325 as well has been examined by determining the chloramphenicol resistance and studying the enzyme activity of Cm-acetylase. A high level of enzyme synthesis is connected with transcription from PL, PR and tac promoters.

Translated title of the contribution	Expression of the chloramphenicol acetyltransferase gene is under control of various promoters of E. coli and phage lambda
Original language	Russian
Pages (from-to)	9-16
Number of pages	8
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	4
State	Published - Apr 1986

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title = "Ekspressiia gena khloramfenikolatsetiltransferazy pod kontrolem nekotorykh promotorov E. coli i bakteriofaga lambda.",

abstract = "Plasmids have been constructed with the structural region of the cat gene being under the control of the lactose (lacUV5), tryptophane (trpOP), operons of Escherichia coli, the hybrid trp-lac (tac) promoter and early bacteriophage lambda promoters (PL, PR and PLIT). The expression of chloramphenicolacetyltransferase gene in Escherichia coli cells harbouring such recombinant plasmids and pBR325 as well has been examined by determining the chloramphenicol resistance and studying the enzyme activity of Cm-acetylase. A high level of enzyme synthesis is connected with transcription from PL, PR and tac promoters.",

author = "Mashko, {S. V.} and Podkovyrov, {S. M.} and Trukhan, {M. E.} and Gorovits, {R. L.} and Lebedeva, {M. I.}",

year = "1986",

month = apr,

language = "רוסית",

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AU - Mashko, S. V.

AU - Podkovyrov, S. M.

AU - Trukhan, M. E.

AU - Gorovits, R. L.

AU - Lebedeva, M. I.

PY - 1986/4

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Ekspressiia gena khloramfenikolatsetiltransferazy pod kontrolem nekotorykh promotorov E. coli i bakteriofaga lambda.

Abstract

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