Electrical contacting of redox enzymes by means of oligoaniline-cross- linked enzyme/Carbon nanotube composites

The electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and thioaniline-modified glucose oxidase (GOx) on a thioaniline monolayer-modified Au electrode yields a CNTs/GOx composite cross-linked by the redox-active bisaniline units. The three-dimensional conductivity of the CNTs matrix and the electron-transfer (relay) properties of the bisaniline components lead to the effective electrical wiring of the enzyme units with the electrode, and to the efficient bioelectrocatalytic oxidation of glucose (turnover rate ca. k_et = 1025 s^-1). The conditions to synthesize the three-dimensional CNTs/GOx composite and the parameters influencing the bioelectrocatalytic functions of the modified electrode are discussed