Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons

R. Oren; R. Sfez; N. Korbakov; K. Shabtai; A. Cohen; H. Erez; A. Dormann; H. Cohen; J. Shappir; M. E. Spira; S. Yitzchaik

doi:10.1163/1568562042368077

Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons

R. Oren, R. Sfez, N. Korbakov, K. Shabtai, A. Cohen, H. Erez, A. Dormann, H. Cohen, J. Shappir, M. E. Spira, S. Yitzchaik^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

In the present contribution we report on a novel route to synthesize 2D-polyaniline (2D-PAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth pattern and adhesion to this conducting substrate that is manifested by the formation of giant lamellipodia. The lamellipodial domains are characterized by small gap between the plasma membrane and the 2D-PAN substrate (ca. 30 nm) and actin rich skeleton resembling the skeleton of growth cones. This behavior is characteristic to uniform substrates containing only 2D-PAN. However, in patterned substrates containing additionally poly(L-lysine) Aplysia neurons prefer to extend new neurites on the poly(L-lysine) domains.

Original language	English
Pages (from-to)	1355-1374
Number of pages	20
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	15
Issue number	11
DOIs	https://doi.org/10.1163/1568562042368077
State	Published - 2004

Bibliographical note

Funding Information:
The project is funded by the European Union Fifth Program contract number: IST-1999-29091 (NEUMIC). R.S. thanks the Israeli ministry of science for an Eshkol post-doctoral fellowship. Part of the research was carried out in the Charles E. Smith Family Laboratory for Collaborative Research in Psychobiology. M.E.S. is the Levi DeViali Prof. in neurobiology. R.O. and R.S. contributed equally to the paper.

Keywords

2D-polyaniline (2D-PAN)
Cultured Aplysia neurons
Micro-contact printing (μCp)
Self-assembled monolayers
Surface modifications

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10.1163/1568562042368077

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title = "Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons",

abstract = "In the present contribution we report on a novel route to synthesize 2D-polyaniline (2D-PAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth pattern and adhesion to this conducting substrate that is manifested by the formation of giant lamellipodia. The lamellipodial domains are characterized by small gap between the plasma membrane and the 2D-PAN substrate (ca. 30 nm) and actin rich skeleton resembling the skeleton of growth cones. This behavior is characteristic to uniform substrates containing only 2D-PAN. However, in patterned substrates containing additionally poly(L-lysine) Aplysia neurons prefer to extend new neurites on the poly(L-lysine) domains.",

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author = "R. Oren and R. Sfez and N. Korbakov and K. Shabtai and A. Cohen and H. Erez and A. Dormann and H. Cohen and J. Shappir and Spira, {M. E.} and S. Yitzchaik",

note = "Funding Information: The project is funded by the European Union Fifth Program contract number: IST-1999-29091 (NEUMIC). R.S. thanks the Israeli ministry of science for an Eshkol post-doctoral fellowship. Part of the research was carried out in the Charles E. Smith Family Laboratory for Collaborative Research in Psychobiology. M.E.S. is the Levi DeViali Prof. in neurobiology. R.O. and R.S. contributed equally to the paper.",

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AU - Sfez, R.

AU - Korbakov, N.

AU - Shabtai, K.

AU - Cohen, A.

AU - Erez, H.

AU - Dormann, A.

AU - Cohen, H.

AU - Shappir, J.

AU - Spira, M. E.

AU - Yitzchaik, S.

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PY - 2004

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Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons

Abstract

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Keywords

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