Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons

R. Oren, R. Sfez, N. Korbakov, K. Shabtai, A. Cohen, H. Erez, A. Dormann, H. Cohen, J. Shappir, M. E. Spira, S. Yitzchaik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

In the present contribution we report on a novel route to synthesize 2D-polyaniline (2D-PAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth pattern and adhesion to this conducting substrate that is manifested by the formation of giant lamellipodia. The lamellipodial domains are characterized by small gap between the plasma membrane and the 2D-PAN substrate (ca. 30 nm) and actin rich skeleton resembling the skeleton of growth cones. This behavior is characteristic to uniform substrates containing only 2D-PAN. However, in patterned substrates containing additionally poly(L-lysine) Aplysia neurons prefer to extend new neurites on the poly(L-lysine) domains.

Original languageAmerican English
Pages (from-to)1355-1374
Number of pages20
JournalJournal of Biomaterials Science, Polymer Edition
Volume15
Issue number11
DOIs
StatePublished - 2004

Bibliographical note

Funding Information:
The project is funded by the European Union Fifth Program contract number: IST-1999-29091 (NEUMIC). R.S. thanks the Israeli ministry of science for an Eshkol post-doctoral fellowship. Part of the research was carried out in the Charles E. Smith Family Laboratory for Collaborative Research in Psychobiology. M.E.S. is the Levi DeViali Prof. in neurobiology. R.O. and R.S. contributed equally to the paper.

Keywords

  • 2D-polyaniline (2D-PAN)
  • Cultured Aplysia neurons
  • Micro-contact printing (μCp)
  • Self-assembled monolayers
  • Surface modifications

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