TY - JOUR
T1 - Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons
AU - Oren, R.
AU - Sfez, R.
AU - Korbakov, N.
AU - Shabtai, K.
AU - Cohen, A.
AU - Erez, H.
AU - Dormann, A.
AU - Cohen, H.
AU - Shappir, J.
AU - Spira, M. E.
AU - Yitzchaik, S.
PY - 2004
Y1 - 2004
N2 - In the present contribution we report on a novel route to synthesize 2D-polyaniline (2D-PAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth pattern and adhesion to this conducting substrate that is manifested by the formation of giant lamellipodia. The lamellipodial domains are characterized by small gap between the plasma membrane and the 2D-PAN substrate (ca. 30 nm) and actin rich skeleton resembling the skeleton of growth cones. This behavior is characteristic to uniform substrates containing only 2D-PAN. However, in patterned substrates containing additionally poly(L-lysine) Aplysia neurons prefer to extend new neurites on the poly(L-lysine) domains.
AB - In the present contribution we report on a novel route to synthesize 2D-polyaniline (2D-PAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth pattern and adhesion to this conducting substrate that is manifested by the formation of giant lamellipodia. The lamellipodial domains are characterized by small gap between the plasma membrane and the 2D-PAN substrate (ca. 30 nm) and actin rich skeleton resembling the skeleton of growth cones. This behavior is characteristic to uniform substrates containing only 2D-PAN. However, in patterned substrates containing additionally poly(L-lysine) Aplysia neurons prefer to extend new neurites on the poly(L-lysine) domains.
KW - 2D-polyaniline (2D-PAN)
KW - Cultured Aplysia neurons
KW - Micro-contact printing (μCp)
KW - Self-assembled monolayers
KW - Surface modifications
UR - http://www.scopus.com/inward/record.url?scp=10644282263&partnerID=8YFLogxK
U2 - 10.1163/1568562042368077
DO - 10.1163/1568562042368077
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C2 - 15648568
AN - SCOPUS:10644282263
SN - 0920-5063
VL - 15
SP - 1355
EP - 1374
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 11
ER -