Electrocatalytic reduction of organic peroxides in organic solvents by microperoxidase-11 immobilized as a monolayer on a gold electrode

Andrew N.J. Moore, Eugenii Katz, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

The heme-containing undecapeptide microperoxidase-11 was immobilized as a monolayer on gold wire electrodes by carbodiimide coupling of the peptide to a cystamine monolayer. Direct communication between the electrode and the heme center was observed in acetonitrile and ethanol. The observed formal potential for the FeIII/FeII couple for the immobilized microperoxidase-11 was -0.290 V vs. SCE in acetonitrile and -0.286 V in ethanol. These electrodes were applied as amperometric sensors for organic peroxides in acetonitrile and ethanol. The electrocatalytic reduction of cumene hydroperoxide, t-butyl hydroperoxide and hydrogen peroxide was demonstrated. In acetonitrile, kinetic parameters (Km and Imax) were evaluated using a modified Michaelis-Menten analysis for the electrocatalysis of the reduction of cumene hydroperoxide, t-butyl hydroperoxide and hydrogen peroxide.

Original languageEnglish
Pages (from-to)189-192
Number of pages4
JournalJournal of Electroanalytical Chemistry
Volume417
Issue number1-2
DOIs
StatePublished - 7 Nov 1996

Keywords

  • Electrocatalysis
  • Gold electrode
  • Microperoxidase-11
  • Monolayer
  • Organic peroxides
  • Organic solvents

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