Abstract
The heme-containing undecapeptide microperoxidase-11 was immobilized as a monolayer on gold wire electrodes by carbodiimide coupling of the peptide to a cystamine monolayer. Direct communication between the electrode and the heme center was observed in acetonitrile and ethanol. The observed formal potential for the FeIII/FeII couple for the immobilized microperoxidase-11 was -0.290 V vs. SCE in acetonitrile and -0.286 V in ethanol. These electrodes were applied as amperometric sensors for organic peroxides in acetonitrile and ethanol. The electrocatalytic reduction of cumene hydroperoxide, t-butyl hydroperoxide and hydrogen peroxide was demonstrated. In acetonitrile, kinetic parameters (Km and Imax) were evaluated using a modified Michaelis-Menten analysis for the electrocatalysis of the reduction of cumene hydroperoxide, t-butyl hydroperoxide and hydrogen peroxide.
Original language | English |
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Pages (from-to) | 189-192 |
Number of pages | 4 |
Journal | Journal of Electroanalytical Chemistry |
Volume | 417 |
Issue number | 1-2 |
DOIs | |
State | Published - 7 Nov 1996 |
Keywords
- Electrocatalysis
- Gold electrode
- Microperoxidase-11
- Monolayer
- Organic peroxides
- Organic solvents