TY - JOUR
T1 - Electrochemical detection of superoxide anions in HeLa cells by using two enzyme-free sensors prepared from ZIF-8-derived carbon nanomaterials
AU - Li, Yufei
AU - Zhang, Huanhuan
AU - Cai, Xuan
AU - Zhao, Hongli
AU - Magdassi, Shlomo
AU - Lan, Minbo
N1 - Publisher Copyright:
© 2019, Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Two kinds of carbon-based nanozymes were constructed from the same precursor of zeolitic imidazolate framework-8 (ZIF-8) for O2•− determination. Hollow carbon cubic nanomaterial (labelled as HCC) was obtained by chemically etching ZIF-8 with tannic acid and a subsequent calcination. A porous carbon cubic nanomaterial (labelled as PCC) was prepared by directly pyrolysis. Then HCC and PCC were immobilized on the surface of screen printed carbon electrodes (SPCE), fabricating HCC and PCC modified electrodes (denoted as HCC/SPCE and PCC/SPCE). HCC/SPCE, best operated at −0.5 V (vs. Ag/AgCl), has a sensitivity of 6.55 × 102 nA μM−1 cm−2 with a detection limit of 207 nM (at S/N = 3) for O2•− sensing. And PCC/SPCE, best operated at −0.4 V (vs. Ag/AgCl), exhibited a superior performance for O2•− detection with a sensitivity of 1.14 × 103 nA μM−1 cm−2 and a low detection limit of 140 nM (at S/N = 3). The two sensors possess excellent reproducibility and stability. They were used to sense O2•− released from HeLa cells. [Figure not available: see fulltext.].
AB - Two kinds of carbon-based nanozymes were constructed from the same precursor of zeolitic imidazolate framework-8 (ZIF-8) for O2•− determination. Hollow carbon cubic nanomaterial (labelled as HCC) was obtained by chemically etching ZIF-8 with tannic acid and a subsequent calcination. A porous carbon cubic nanomaterial (labelled as PCC) was prepared by directly pyrolysis. Then HCC and PCC were immobilized on the surface of screen printed carbon electrodes (SPCE), fabricating HCC and PCC modified electrodes (denoted as HCC/SPCE and PCC/SPCE). HCC/SPCE, best operated at −0.5 V (vs. Ag/AgCl), has a sensitivity of 6.55 × 102 nA μM−1 cm−2 with a detection limit of 207 nM (at S/N = 3) for O2•− sensing. And PCC/SPCE, best operated at −0.4 V (vs. Ag/AgCl), exhibited a superior performance for O2•− detection with a sensitivity of 1.14 × 103 nA μM−1 cm−2 and a low detection limit of 140 nM (at S/N = 3). The two sensors possess excellent reproducibility and stability. They were used to sense O2•− released from HeLa cells. [Figure not available: see fulltext.].
KW - Carbon-based nanozymes
KW - Metal-organic frameworks
KW - Non-enzymatic sensors
KW - Screen printed carbon electrodes
KW - Zeolitic imidazolate framework
UR - http://www.scopus.com/inward/record.url?scp=85066153308&partnerID=8YFLogxK
U2 - 10.1007/s00604-019-3473-y
DO - 10.1007/s00604-019-3473-y
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C2 - 31119470
AN - SCOPUS:85066153308
SN - 0026-3672
VL - 186
JO - Microchimica Acta
JF - Microchimica Acta
IS - 6
M1 - 370
ER -