Electrochemically controlled drug-mimicking protein release from iron-alginate thin-films associated with an electrode

Zhiyuan Jin, Güray Güven, Vera Bocharova*, Jan Halámek, Ihor Tokarev, Sergiy Minko, Artem Melman, Daniel Mandler, Evgeny Katz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

Novel biocompatible hybrid-material composed of iron-ion-cross-linked alginate with embedded protein molecules has been designed for the signal-triggered drug release. Electrochemically controlled oxidation of Fe 2+ ions in the presence of soluble natural alginate polymer and drug-mimicking protein (bovine serum albumin, BSA) results in the formation of an alginate-based thin-film cross-linked by Fe 3+ ions at the electrode interface with the entrapped protein. The electrochemically generated composite thin-film was characterized by electrochemistry and atomic force microscopy (AFM). Preliminary experiments demonstrated that the electrochemically controlled deposition of the protein-containing thin-film can be performed at microscale using scanning electrochemical microscopy (SECM) as the deposition tool producing polymer-patterned spots potentially containing various entrapped drugs. Application of reductive potentials on the modified electrode produced Fe 2+ cations which do not keep complexation with alginate, thus resulting in the electrochemically triggered thin-film dissolution and the protein release. Different experimental parameters, such as the film-deposition time, concentrations of compounds and applied potentials, were varied in order to demonstrate that the electrodepositon and electrodissolution of the alginate composite film can be tuned to the optimum performance. A statistical modeling technique was applied to find optimal conditions for the formation of the composite thin-film for the maximal encapsulation and release of the drug-mimicking protein at the lowest possible potential.

Original languageAmerican English
Pages (from-to)466-475
Number of pages10
JournalACS applied materials & interfaces
Volume4
Issue number1
DOIs
StatePublished - 25 Jan 2012

Keywords

  • alginate
  • drug release
  • electrochemical control
  • modified electrode
  • stimuli-responsive electrode

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