TY - JOUR
T1 - Electron-impact and chemical ionization detection of nicotine and cotinine by gas chromatography-mass spectrometry in rat plasma and brain
AU - Deutsch, Joseph
AU - Hegedus, Lajos
AU - Greig, Nigel H.
AU - Rapoport, Stanley I.
AU - Soncrant, Timothy T.
PY - 1992/8/7
Y1 - 1992/8/7
N2 - Nicotine and its metabolite, cotinine, were measured in rat plasma and brain by gas chromatography-mass spectrometry. Both agents were extracted from plasma and brain, separated on a capillary column, and quantified by single-ion monitoring. The major fragment ions of nicotine and cotinine at m/z 84 and m/z 98, respectively, were monitored by electron-impact ionization detection and the protonated molecular ions at m/z 163 and m/z 177, respectively, were monitored by chemical ionization detection. Both compounds were quantified using deuterium-labeled nicotine and cotinine, respectively, as internal standards.
AB - Nicotine and its metabolite, cotinine, were measured in rat plasma and brain by gas chromatography-mass spectrometry. Both agents were extracted from plasma and brain, separated on a capillary column, and quantified by single-ion monitoring. The major fragment ions of nicotine and cotinine at m/z 84 and m/z 98, respectively, were monitored by electron-impact ionization detection and the protonated molecular ions at m/z 163 and m/z 177, respectively, were monitored by chemical ionization detection. Both compounds were quantified using deuterium-labeled nicotine and cotinine, respectively, as internal standards.
UR - http://www.scopus.com/inward/record.url?scp=0026643739&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(92)80366-X
DO - 10.1016/0378-4347(92)80366-X
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C2 - 1447354
AN - SCOPUS:0026643739
SN - 0378-4347
VL - 579
SP - 93
EP - 98
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -