Electron-impact and chemical ionization detection of nicotine and cotinine by gas chromatography-mass spectrometry in rat plasma and brain

Joseph Deutsch, Lajos Hegedus, Nigel H. Greig, Stanley I. Rapoport, Timothy T. Soncrant*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Nicotine and its metabolite, cotinine, were measured in rat plasma and brain by gas chromatography-mass spectrometry. Both agents were extracted from plasma and brain, separated on a capillary column, and quantified by single-ion monitoring. The major fragment ions of nicotine and cotinine at m/z 84 and m/z 98, respectively, were monitored by electron-impact ionization detection and the protonated molecular ions at m/z 163 and m/z 177, respectively, were monitored by chemical ionization detection. Both compounds were quantified using deuterium-labeled nicotine and cotinine, respectively, as internal standards.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume579
Issue number1
DOIs
StatePublished - 7 Aug 1992
Externally publishedYes

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