TY - JOUR
T1 - Electron-impact ionization detection of scopolamine by gas chromatography-mass spectrometry in rat plasma and brain
AU - Deutsch, Joseph
AU - Soncrant, Timothy T.
AU - Greig, Nigel H.
AU - Rapoport, Stanley I.
PY - 1990
Y1 - 1990
N2 - Scopolamine, a muscarinic receptor antagonist, was measured in rat plasma and brain using gas chromatography-mass spectrometry with electron-impact ionization detection. Extracted scopolamine was either directly derivatized or first hydrolyzed to scopine, then derivatized with heptafluorobutyric anhydride, separated on a capillary column and quantified by mass fragmentography. Electron-impact ionization produced a common fragment peak at m z 138 that was monitored along with trideuterated scopolamine, an internal standard ( m z 141). The method can be used to measure scopolamine concentrations of 2 ng/ml in rat plasma and 20 ng/g in rat brain.
AB - Scopolamine, a muscarinic receptor antagonist, was measured in rat plasma and brain using gas chromatography-mass spectrometry with electron-impact ionization detection. Extracted scopolamine was either directly derivatized or first hydrolyzed to scopine, then derivatized with heptafluorobutyric anhydride, separated on a capillary column and quantified by mass fragmentography. Electron-impact ionization produced a common fragment peak at m z 138 that was monitored along with trideuterated scopolamine, an internal standard ( m z 141). The method can be used to measure scopolamine concentrations of 2 ng/ml in rat plasma and 20 ng/g in rat brain.
UR - http://www.scopus.com/inward/record.url?scp=0025311611&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)82391-9
DO - 10.1016/S0378-4347(00)82391-9
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C2 - 2384572
AN - SCOPUS:0025311611
SN - 0378-4347
VL - 528
SP - 325
EP - 331
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -