Electron-impact ionization detection of scopolamine by gas chromatography-mass spectrometry in rat plasma and brain

Joseph Deutsch*, Timothy T. Soncrant, Nigel H. Greig, Stanley I. Rapoport

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Scopolamine, a muscarinic receptor antagonist, was measured in rat plasma and brain using gas chromatography-mass spectrometry with electron-impact ionization detection. Extracted scopolamine was either directly derivatized or first hydrolyzed to scopine, then derivatized with heptafluorobutyric anhydride, separated on a capillary column and quantified by mass fragmentography. Electron-impact ionization produced a common fragment peak at m z 138 that was monitored along with trideuterated scopolamine, an internal standard ( m z 141). The method can be used to measure scopolamine concentrations of 2 ng/ml in rat plasma and 20 ng/g in rat brain.

Original languageEnglish
Pages (from-to)325-331
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume528
Issue numberC
DOIs
StatePublished - 1990
Externally publishedYes

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