TY - JOUR
T1 - Electron microscopic evidence for splicing of moloney murine leukemia virus RNAs
AU - Panet, Amos
AU - Gorecki, Marian
AU - Bratosin, Susan
AU - Aloni, Yosef
PY - 1978/9
Y1 - 1978/9
N2 - Poly (A) containing RNA extracted from Moloney murine leukemia virus infected mouse cells was hybridized with long single-stranded complementary DNA, prepared in detergent disrupted virions. Visualization of the hybrids in the electron microscope revealed among the structures, circles and circles with tails. Measurements performed on the circular molecules revealed two major species with circumferences corresponding to 3 and 8.2 kilobases. The latter structures had identical size to circles obtained after annealing ot cDNA with the viral genome, 35S RNA. Circularization of a small viral RNA (3 kb) from infected cells in the RNA-cDNA hybrids is a direct evidence that like the 35S RNA it shares similar nucleotide sequences at both the 5′ and 3′ ends. The presence of 5′ end sequences common to the two RNA species indicates the existence of a spliced viral RNA. Furthermore, based on the circularization of viral RNA in the hybrids, we suggest a new way to quantitate and determine the lengths of spliced RNA in retrovirus infected cells.
AB - Poly (A) containing RNA extracted from Moloney murine leukemia virus infected mouse cells was hybridized with long single-stranded complementary DNA, prepared in detergent disrupted virions. Visualization of the hybrids in the electron microscope revealed among the structures, circles and circles with tails. Measurements performed on the circular molecules revealed two major species with circumferences corresponding to 3 and 8.2 kilobases. The latter structures had identical size to circles obtained after annealing ot cDNA with the viral genome, 35S RNA. Circularization of a small viral RNA (3 kb) from infected cells in the RNA-cDNA hybrids is a direct evidence that like the 35S RNA it shares similar nucleotide sequences at both the 5′ and 3′ ends. The presence of 5′ end sequences common to the two RNA species indicates the existence of a spliced viral RNA. Furthermore, based on the circularization of viral RNA in the hybrids, we suggest a new way to quantitate and determine the lengths of spliced RNA in retrovirus infected cells.
UR - http://www.scopus.com/inward/record.url?scp=0018123780&partnerID=8YFLogxK
U2 - 10.1093/nar/5.9.3219
DO - 10.1093/nar/5.9.3219
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C2 - 704353
AN - SCOPUS:0018123780
SN - 0305-1048
VL - 5
SP - 3219
EP - 3230
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 9
ER -