Electron spin resonance measurements of the effect of lonophores on the transmembrane pH gradient of an acidophilic bacterium

D. McLaggan, S. Belkin, L. Packer, A. Matin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The ΔpH in ionophore-treated cells of an acidophile has been determined by electron spin resonance spectroscopy. The values obtained were comparable to those obtained using the more conventional techniques involving radiolabeled probes. No binding of the spin-labeled probe was observed as determined by two independent control experiments and by the characteristics of the probe signal. These results led us to conclude that the ΔpH measured in protonophore/ionophore-treated cells is a result of a Donnan potential, which may be a physical property of all intact bacterial cells at low pH values.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume273
Issue number1
DOIs
StatePublished - 15 Aug 1989
Externally publishedYes

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