Abstract
The ΔpH in ionophore-treated cells of an acidophile has been determined by electron spin resonance spectroscopy. The values obtained were comparable to those obtained using the more conventional techniques involving radiolabeled probes. No binding of the spin-labeled probe was observed as determined by two independent control experiments and by the characteristics of the probe signal. These results led us to conclude that the ΔpH measured in protonophore/ionophore-treated cells is a result of a Donnan potential, which may be a physical property of all intact bacterial cells at low pH values.
Original language | English |
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Pages (from-to) | 206-214 |
Number of pages | 9 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 273 |
Issue number | 1 |
DOIs | |
State | Published - 15 Aug 1989 |
Externally published | Yes |