TY - JOUR
T1 - Electronic transduction of polymerase or reverse transcriptase induced replication processes on surfaces
T2 - Highly sensitive and specific detection of viral genomes
AU - Patolsky, Fernando
AU - Lichtenstein, Amir
AU - Kotler, Moshe
AU - Willner, Itamar
PY - 2001/6/18
Y1 - 2001/6/18
N2 - Replication, precipitation, and amplification: Polymerase or reverse transcriptase induced replication of DNA/RNA on a transducer (electrode or piezoelectric crystal) leads to the ultrasensitive specific electronic transduction of viral genomes. Biotin tags (B) on the double-stranded assembly provide docking sites for a conjugate between avidin (A) and an alkaline phosphatase (AP). Enzyme biocatalysis of substrate (S) to the insoluble product (P), which precipitates onto the transducer (yellow surface), provides amplification in the analysis of the target DNA.
AB - Replication, precipitation, and amplification: Polymerase or reverse transcriptase induced replication of DNA/RNA on a transducer (electrode or piezoelectric crystal) leads to the ultrasensitive specific electronic transduction of viral genomes. Biotin tags (B) on the double-stranded assembly provide docking sites for a conjugate between avidin (A) and an alkaline phosphatase (AP). Enzyme biocatalysis of substrate (S) to the insoluble product (P), which precipitates onto the transducer (yellow surface), provides amplification in the analysis of the target DNA.
KW - Biosensors
KW - DNA replication
KW - Impedance spectroscopy
KW - Quartz-crystal microbalance
KW - RNA replication
UR - http://www.scopus.com/inward/record.url?scp=0035907817&partnerID=8YFLogxK
U2 - 10.1002/1521-3773(20010618)40:12<2261::AID-ANIE2261>3.0.CO;2-P
DO - 10.1002/1521-3773(20010618)40:12<2261::AID-ANIE2261>3.0.CO;2-P
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AN - SCOPUS:0035907817
SN - 1433-7851
VL - 40
SP - 2261
EP - 2265
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 12
ER -