Abstract
A new matrix for electrophoresis, a low molecular weight organogel, is described. Dansylated amino acids and peptides were separated by planar and capillary electrophoresis in acetonitrile gels of trans-(1S,2S)-1,2-bis- (dodecylamido)cyclohexane. The superior separation ability of the organogel over its corresponding buffer solution in capillary electrophoresis is illustrated. Organogels provide all the advantages associated with planar matrixes with 100% efficient recovery and transfer of the analytes to a mass spectrometer. We demonstrate that the planar gel can be liquefied and injected as is into an ESI-MS to identify the separands.
Original language | English |
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Pages (from-to) | 5399-5404 |
Number of pages | 6 |
Journal | Analytical Chemistry |
Volume | 76 |
Issue number | 18 |
DOIs | |
State | Published - 15 Sep 2004 |