Electrophoretic analysis of reduced protein fractions from a new South African high-lysine (opaque-2) hybrid and three other opaque-like maize types.

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the reduced polypeptides of the major endosperm protein fractions of drought-damaged and normal white dent maize, a high lysine hybrid and waxy and floury types of maize did not provide clear-cut distinctions between the types apart from the lower intensity and more diffuse nature of the 25 kilodalton reduced zein polypeptide from the high lysine hybrid. The greatest divergence between the 5 types occurred in the Landry-Moureaux fraction IV preparations.<new para>ADDITIONAL ABSTRACT:<new para>In Landry-Moureaux Fraction II (LMII) preparations, the band corresponding to the 25 kilodalton reduced zein polypeptide was more diffuse and more faintly stained for the white-grained, high-lysine hybrid (HL1) than for normal-grained and drought-damaged white dent lines, a waxy cultivar and a floury line. The greatest divergence between the five types occurred in LMIV preparations.